The genome organization, gene structure, and host range of five podoviruses that infect Ralstonia solanacearum, the causative agent of bacterial wilt disease were characterized. The phages fell into two distinctive groups based on the genome position of the RNA polymerase gene (i.e., T7-type and ϕKMV-type). One-step growth experiments revealed that ϕRSB2 (a T7-like phage) lysed host cells more ...

Phosphine is shown to catalyse the reaction of CO2 with 9-BBN to give mixtures of HCO2(B(C8H14)) 3, H2C(OB(C8H14))24 and MeOB(C8H14) 5; at 0.02 mol% of tBu3P 5 is obtained in 98% yield at 60 °C with TON of almost 5500 and a TOF of 170. Under stoichiometric conditions the species (R3PCH2O)(HC(O)O)B(C8H14) (R = tBu 1,4-MeC6H42) were isolated and characterized.

We investigate the possibility of distinguishing standard model from composite models at an intermediate energy range (45 60 GeV). We show that effects due to multiple neutral weak bosons and their finite widths can be observable in e+e-+ p+pforward-backward asymmetry. Submitted to Physical Review D *Work supported in part by the Department of Energy, contract DEAC03-76SF00515 and by Centre Nat...

A pilot field study was conducted at the Jagjeetpur Municipal Sewage treatment plant situated in the Haridwar town in Uttarakhand state, India. The objectives of the present study were to study the effect of treated wastewater on the production of various paddy varieties (Sharbati, PR-114, PB-1, Menaka, PB1121 and PB 1509) and the emission of GHG gases (CO2, CH4 and N2O) as compared to the same...

Blastocystis cysts were detected in 38% (47/123) (37 Scottish, 17 Malaysian) of sewage treatment works. Fifty percent of influents (29% Scottish, 76% Malaysian) and 28% of effluents (9% Scottish, 60% Malaysian) contained viable cysts. Viable cysts, discharged in effluent, provide further evidence for the potential for waterborne transmission of Blastocystis.

Four T7 products, DNA polymerase, gene 4 protein, RNA polymerase, and DNA binding protein, have been purified from phage-infected cells. It has been previously shown (Hinkle, D. C., and Richardson, C. C. (1975) J. Biol. Chem 250, 5523-5529; Kolodner, R., and Richardson, C, C. (1978) J. Biol. Chem 253, 574-584) that two T7 products, DNA polymerase and gene 4 protein, catalyze extensive synthesis...

A mutant of Salmonella typhimurium was found to be sensitive to killing by coliphage T7 because of an alteration in its surface properties. However, the infections were abortive and studies with (32)P-labeled T7 grown in Escherichia coli B (T7.B) indicated that the phage DNA was restricted by S. typhimurium. When a mutant T7 which survived the restriction and produced plaques on Salmonella (T7....

Soil fertility is one of the most promising factors that directly impact sustainable crop production. Therefore, a pot experiment was conducted in net house on capsicum (Capsicum annuum L.) agricultural soil to study effects vermicompost (VC) and inorganic fertilizers (NPK) growth, yield nutrient content plants. The growth parameters nutritional values were observed during under eleven treatmen...

This investigation aimed to assess the influence of soil-applied vermicompost, nitrogen and zinc on vegetative growth parameters, yield, mineral nutrient concentration fruits okra (Abelmoschus esculentus L. Moench). The experiment was laid out in a Completely Randomized Design followed by three replications. Data were subjected analysis variance mean values compared using Tukey’s Range Test at ...

The synthesis of living entities in the laboratory is a standing challenge that calls for innovative approaches. Using a cell-free transcription-translation system as a molecular programming platform, we show that the bacteriophage T7, encoded by a 40 kbp DNA program composed of about 60 genes, can be entirely synthesized from its genomic DNA in a test tube reaction. More than a billion infecti...