Ribonucleases (RNases) are ubiquitous enzymes that can detrimentally affect RNA isolation in the laboratory. Many applications such as RT-PCR and in vitro transcription and translation may be affected as well. RNase can renature after autoclaving and is difficult to inactivate. Many RNase inhibitors are available commercially. Promega’s RNasin® Ribonuclease Inhibitor, one of the first introduce...

Human RNase P is implicated in transcription of small non-coding RNA genes by RNA polymerase III (Pol III), but the precise role of this ribonucleoprotein therein remains unknown. We here show that targeted destruction of HeLa nuclear RNase P inhibits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation complex formation. Biochemical purification analy...

Upon introducing charge carriers into the copper-oxygen sheets of the enigmatic lamellar cuprates, the ground state evolves from an insulator to a superconductor and eventually to a seemingly conventional metal (a Fermi liquid). Much has remained elusive about the nature of this evolution and about the peculiar metallic state at intermediate hole-carrier concentrations (p). The planar resistivi...

A simple matrix is a (0,1)-matrix with no repeated columns. For a (0,1)-matrix F , we define that a (0,1)-matrix A has F as a configuration if there is a submatrix of A which is a row and column permutation of F (trace is the set system version of a configuration). Let |A| denote the number of columns of A. We define forb(m,F ) = max{|A| : A is m-rowed simple matrix and has no configuration F}....