نتایج جستجو برای: synthetic promoter

تعداد نتایج: 228934  

2016
Ning Zhang Leah K. McHale John J. Finer

Introns, especially the first intron in the 5' untranslated region (5'UTR), can significantly impact gene expression via intron-mediated enhancement (IME). In this study, we demonstrate the leader intron of a soybean elongation factor 1A (eEF1A) gene (GmScreamM8) was essential for the high activity of the native promoter. Furthermore, the interaction of the GmScreamM8 leader intron with regulat...

2014
A. Malcolm Campbell Todd Eckdahl Brian Cronk Corinne Andresen Paul Frederick Samantha Huckuntod Claire Shinneman Annie Wacker Jason Yuan

The Vision and Change report recommended genuine research experiences for undergraduate biology students. Authentic research improves science education, increases the number of scientifically literate citizens, and encourages students to pursue research. Synthetic biology is well suited for undergraduate research and is a growing area of science. We developed a laboratory module called pClone t...

Journal: :Journal of virology 1992
N Yanagida R Ogawa Y Li L F Lee K Nazerian

Two Marek's disease virus (MDV) genes, one homologous to the glycoprotein B gene of herpes simplex virus and encoding the B antigen complex and the other encoding a 38-kDa phosphorylated protein (pp38), were inserted into the fowlpox virus (FPV) genome under the control of poxvirus promoters. Randomly selected nonessential regions of FPV were used for insertion, and the vaccinia virus 7.5 kDa p...

2013
Fabio Rusconi Fabio Simeoni Priscilla Francia Eleonora Cominelli Lucio Conti Matteo Riboni Laura Simoni Cathie R. Martin Chiara Tonelli Massimo Galbiati

Plants have evolved different strategies to resist drought, of which the best understood is the abscisic acid (ABA)-induced closure of stomatal pores to reduce water loss by transpiration. The availability of useful promoters that allow for precise spatial and temporal control of gene expression in stomata is essential both for investigating stomatal regulation in model systems and for biotechn...

2017
Adam J. Brown Suzanne J. Gibson Diane Hatton David C. James

Comprehensive de novo-design of complex mammalian promoters is restricted by unpredictable combinatorial interactions between constituent transcription factor regulatory elements (TFREs). In this study, we show that modular binding sites that do not function cooperatively can be identified by analyzing host cell transcription factor expression profiles, and subsequently testing cognate TFRE act...

Journal: :ACS synthetic biology 2016
Gianfranco Fiore Giansimone Perrino Mario di Bernardo Diego di Bernardo

Real-time automatic regulation of gene expression is a key technology for synthetic biology enabling, for example, synthetic circuit's components to operate in an optimal range. Computer-guided control of gene expression from a variety of inducible promoters has been only recently successfully demonstrated. Here we compared, in silico and in vivo, three different control algorithms: the Proport...

Journal: :Nucleic acids research 2016
Brady F Cress J Andrew Jones Daniel C Kim Quentin D Leitz Jacob A Englaender Shannon M Collins Robert J Linhardt Mattheos A G Koffas

Robust gene circuit construction requires use of promoters exhibiting low crosstalk. Orthogonal promoters have been engineered utilizing an assortment of natural and synthetic transcription factors, but design of large orthogonal promoter-repressor sets is complicated, labor-intensive, and often results in unanticipated crosstalk. The specificity and ease of targeting the RNA-guided DNA-binding...

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