Studies were undertaken to determine the mechanisms leading to altered mitochondrial function in ischemic myocardium. A new procedure has been developed to routinely isolate 60-70% of the total mitochondrial protein from heart tissue. After 1 hour of ischemia, mitochondria exhibit decreases of more than 50% in phosphorylating respiration for both NADH- and succinate-linked substrates compared t...

fresh semen obtained from infertile men occasionally exhibits the total absence of motile sperm (100%asthenozoospermia). vitality tests may reveal that a proportion of the immotile sperm has functional membranes, which are considered viable. an easy test for the prior determination of sperm viability may assist in sperm selection for the microinjection of oocytes. the main objective of this stu...

the objective of the present study is to develop and investigate the swelling behavior of ph-sensitive superporous hydrogel (sph) and sph composite (sphc). a novel superporous hydrogel containing poly (methacrylic acid-co-acrylamide) was synthesized from methacrylic acid and acrylamide through the aqueous solution polymerization, using n,n-methylenebisacrylamide as a crosslinker and ammonium pe...

semen analysis, sperm dna fragmentation (sdf) and hypo-osmotic swelling test (host) are usually performed for the evaluation of sperm fertilizing ability. there are some debates over the necessity of sdf and host incorporation in male infertility work-up.semen of 77 men was evaluated by sdf and host through three semen analyses. sperm parameters were arranged into different categories: < 5%, 5-...

the transport properties of liquids and gases through polymeric materialsplay a very important role in some areas of industrial applications. in thisstudy, natural rubber (nr)/caco3 composites were prepared by melt mixingmethod. by equilibrium swelling test, the transport process of toluene in the prepared natural rubber composites was investigated. the diffusion and transport of toluene throug...

pH-sensitive hydrogels are suitable candidates for oral drug delivery of peptides due to their ability to respond to their environment. We have developed new hydrogels composed of poly(methacrylic acid) (PMAA) grafted with poly(ethylene glycol) (PEG) (P(MAA-g-EG)) which can be used as drug delivery carriers for salmon calcitonin. P(MAA-g-EG) hydrogels were prepared by free radical solution poly...