Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

Callus induction and withanolides production through cell suspension culture of Withania coagulans (Stocks) Dunal

(Simizu et al., 1973; Hashimoto et al., 1975). Infected culture fluids were harvested in 24 hr after infection for preparing a crude virus suspension. The virus suspension was purified according to the procedures described elsewhere (Hashimoto et al., 1975). Physical particles of viruses were counted by the loop-drop method of Watson, Russell and Wildy (1963). One-tenth milliliter of virus susp...

Growing insect cells with high air space in culture vessel is common from the early development of suspension cell culture. We believed and followed it with the hope that it allows sufficient air for optimal cell growth. However, we missed to identify how much air exactly cells need for its growth and multiplication. Here we present the innovative method that changed the way we run insect cell ...

The metabolism of [(14)CH(3)]2-(2,4-dichlorophenoxy)isobutyric acid (DIB) was studied in plants and cell suspension cultures of Lycopersicon esculentum Mill. sp. ;Lukullus'. Both plants and cells in suspension culture showed a rapid uptake of DIB from nutrient media. The metabolites, isolated by extraction with methanol and separated by chromatographic methods, were identified by enzymic, chemi...

Pectic oligomers induced a rapid, transient increase in ethylene biosynthesis when added to pear cells in suspension culture. The rate of ethylene biosynthesis increased within 30 to 40 minutes after oligomer addition, reached a maximum between 90 and 120 minutes after addition, and then decreased to basal rates of synthesis. Both the rapid increase and decrease in biosynthesis appear to be pre...

BACKGROUND Cell culture conditions can greatly influence the results of nanoparticle (NP) uptake assays. In this study, 10 nm gold nanoparticles (AuNPs) and RAW 264.7 macrophages were used as a model system, while instrumental neutron activation analysis (NAA) was used as the elemental analysis technique to determine AuNP levels produced by the various culturing conditions. Static plate-based a...

Plant cell aggregates have long been implicated in affecting cellular metabolism in suspension culture, yet the rigorous characterization of aggregate size as a process variable and its effect on bioprocess performance has not been demonstrated. Aggregate fractionation and analysis of biomass-associated product is commonly used to assess the effect of aggregation, but we establish that this met...

When murine L1210 leukemia cells growing in suspension culture are deprived of tryptophan, there is a rapid fall in DNA and histone synthesis and a less rapid fall in whole cellular protein and non-histone nuclear protein synthesis. Initially, the S phase of the cell cycle becomes prolonged, but subsequently the cells become defective in their ability to reinitiate S phase. By 24 hr complete gr...