Temperature compensation is a striking feature of the circadian clock. Here we investigate biochemical mechanisms underlying temperature-compensated, CKIδ-dependent multi-site phosphorylation in mammals. We identify two mechanisms for temperature-insensitive phosphorylation at higher temperature: lower substrate affinity to CKIδ-ATP complex and higher product affinity to CKIδ-ADP complex. Inhib...

The 11-kb sequence encompassing the alkylbenzene upper pathway in Pseudomonas putida 01G3, a psychrotrophic strain able to degrade alkylbenzenes at low temperatures, was characterized. Together with a potential regulator (EbdR), six putative enzymes (EbdAaAbAcAdBC) were identified, and they exhibited highly significant similarities with enzymes implicated in the equivalent pathway in P. putida ...

Liquid 4He shows a dewetting phase on Cs and Rb at low temperatures. These are the only substrates which are not wetted by 4He. On Cs the wetting temperature, Tw, is about 2 K. Usually one observes a thick metastable helium film remaining on the Cs substrate when either the surface is cooled below Tw (so starting from a wet film) or when bulk helium (in the form of a drop or an advancing film) ...

The temperature and atomic hydrogen concentration profiles in a hot filament type diamond deposition reactor were determined experimentally and theoretically to demonstrate that the reaction of atomic hydrogen on the substrate surface plays an important role in the heating of the substrate. For a given filament temperature, the substrate temperature in helium was significantly lower than that i...

Coevaporated bismuth-tellurium and antimony-tellurium films were fabricated under various deposition conditions scontrolled evaporation rates of individual species, substrate temperature, and substrate materiald, and their thermoelectric sTEd properties sSeebeck coefficient, electrical resistivity, and carrier concentrationd were measured in search of optimal TE performance. The tellurium atomi...

Top-down control and elasticity analysis was conducted on mitochondria isolated from the midgut of the tobacco hornworm (Manduca sexta) to assess how temperature affects oxidative phosphorylation in a eurythermic ectotherm. Oxygen consumption and protonmotive force (measured as membrane potential in the presence of nigericin) were monitored at 15, 25, and 35 degrees C. State 4 respiration displ...