This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210°C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic® CTec 2 (20 U or 0.87 FPU g(-1) substrate) cellulase plus β-glucosidase (2 U g(-1) substrate) and a yeast inoculum of 10% (v/v or 8.0×10(7) cells mL(-1)). At a subst...

Enzyme catalysis has been studied as a supportive process in biochemical reactions since the 1800s. The conventional paradigm states that a catalyst accelerates a chemical reaction without affecting its equilibrium) outcome. This notion was formed by decades of analysis of biochemical catalysis under the conditions defined by Henri in 1903, which stipulate that enzyme, substrate and the enzyme-...

introduction: antibiotics have the potential to adversely affect the microbial community. for anaerobic digestion, a sufficient methanogenic population needs to be preserved in the system. the main aim of this study was determination of inhibitory concentration of oxytetracycline on methanogenic bacteria. methods: a 120 ml jacketed bioreactor with a 90 ml working volume was inoculated granular ...

A galactoglucomannan oligosaccharide (GGMO) obtained from fiberboard production was evaluated as a dietary supplement for dogs. The GGMO substrate contained increased concentrations of oligosaccharides containing mannose, xylose, and glucose, with the mannose component accounting for 35% of DM. Adult dogs assigned to a 6 × 6 Latin square design were fed 6 diets, each containing a different conc...

The transformation of 3-chlorobenzoate (3CB) and acetate at initial concentrations in the wide range of 10 nM to 16 mM was studied in batch experiments with Pseudomonas sp. strain B13. Transformation rates of 3CB at millimolar concentrations could be described by Michaelis-Menten kinetics (K(infm), 0.13 mM; V(infmax), 24 nmol (middot) mg of protein(sup-1) (middot) min(sup-1)). Experiments with ...

The Goldbeter-Koshland model has been a paradigm for ultrasensitivity in biological networks for more than 30 years. Despite its simplicity the validity of this model is restricted to conditions when the substrate is in excess over the converter enzymes - a condition that is easy to satisfy in vitro, but which is rarely satisfied in vivo. Here, we analyze the Goldbeter-Koshland model by means o...

A continuous-flow apparatus is described for automatically plotting substrate saturation curves, and is suitable for use with a variety of enzymes. A linear concentration gradient of the variable substrate is combined with a fixed proportion of the other substrates and the enzyme, and after passing through a reaction coil the product concentrations are measured spectrophotometrically. Use of a ...

The coupled system of non-linear second-order reaction differential equation in basic enzyme reaction is formulated and closed analytical expressions for substrate and product concentrations are presented. Approximate analytical method (He’s Homotopy perturbation method) is used to solve the coupled non-linear differential equations containing a non-linear term related to enzymatic reaction. Cl...

Previously, we reported that the substrate shape recognition of the Escherichia coli ribonuclease (RNase) P ribozyme depends on the concentration of magnesium ion in vitro. We additionally examined the Bacillus subtilis RNase P ribozyme and found that the B. subtilis enzyme also required high magnesium ion, above 10 mM, for cleavage of a hairpin substrate. The results of kinetic studies showed ...

A chemostat limited by a single growth-limiting substrate displays a rich spectrum of dynamics. Depending on the flow rate and feed concentration, the chemostat settles into a steady state or executes sustained oscillations. The transients in response to abrupt increases in the flow rate or the feed concentration are also quite complex. For example, if the increase in the flow rate is small, th...