Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and seque...

Human papillomavirus (HPV) infection is the leading risk factor for cervical intraepithelial neoplasia (CIN) and cervical cancer. Detection of high-risk HPV infections might identify women who are at increased risk of development or progression of a cervical lesion and may have prognostic significance. Therefore there is considerable interest in identifying and accurately typing the viruses. PC...

background:  aeromonas spp. can cause diarrhea and various infections in humans. access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of aeromonas species. the aim of this study was to evaluate two different methods including api 20e bacterial identification tests and the molecular detection using pcr primers specific for 16s-rrna and 23s-...

Development and evaluation of a single step duplex PCR for simultaneous 1 detection of Fasciola hepatica and Fasciola gigantica 2 (Fasciolidae; Trematoda; Platyhelminthes) 3 Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, 4 Xuyen Thi Kim Le, Chau Thi Minh Hoang, and Nguyen Van De 5 Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and 6 T...

Name of Gene Marker. MPO, myeloperoxidase. Source and Description of Primers. Primers (set A) were designed based on human (Genbank Accession no. D14466) and mouse (Genbank Accession no. X15313) MPO exon 9 and 10 sequences available in the Genbank database. Using porcine genomic DNA as template in the PCR, these primers amplified a 1-kb fragment. Set B primers were designed based on pig DNA seq...

Avoiding complementarity between primers when designing a PCR assay constitutes a central rule strongly anchored in the mind of the molecular scientist. 3'-complementarity will extend the primers during PCR elongation using one another as template, consequently disabling further possible involvement in traditional target amplification. However, a 5'-complementarity will leave the primers unchan...

Evaluating the specificity of polymerase chain reaction (PCR) primers is an essential step in PCR primer design. The MFEprimer-2.0 server allows users to check primer specificity against genomic DNA and messenger RNA/complementary DNA sequence databases quickly and easily. MFEprimer-2.0 uses a k-mer index algorithm to accelerate the search process for primer binding sites and uses thermodynamic...

background molecular farming is the production of important recombinant proteins in transgenic organisms on an agricultural scale. interferons are proteins with antiviral and antitumor activities and can be used for viral infections and cancers treatments. objectives this study reports the transformation of inf α2b gene in tobacco plant for the first time in iran. materials and methods interfer...

An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images ...