The kinetics of the hemolysis induced by filipin is of the damage type, indicating the formation of large nonselective perforations of erythrocyte membranes. The process is relatively independent of the ionic composition of the incubation medium, and the differences between the hemolysis induced by filipin in pig and human erythrocytes are not significant. In a sucrose medium, filipin-induced h...

BACKGROUND Immune-mediated hemolytic anemia (IMHA) is the most common hematologic immune-mediated disease in dogs. Complement fixation on erythrocytes causes hemolysis. Complement inhibition decreases hemolysis in people with the hemolytic disease and also may prove effective in treating IMHA in dogs. HYPOTHESIS/OBJECTIVES Evaluate the in vitro efficacy of 2 complement inhibitors used in huma...

The kinetics of the filipinand amphotericin-B-induced hemolysis of pig, horse and human erythrocytes in isotonic KCl, monoand disaccharide media was investigated. The kinetics of filipininduced hemolysis is of the damage type, with small changes in erythrocyte resistance to the antibiotic as the standard KCl medium is replaced by the saccharide media. The hemolysis induced by amphotericin B is ...

Laboratory values for specimens from a case of intravascular hemolysis showed that hemoglobin was significantly increased and thus could interfere with the determination of other analytes. We studied this problem by adding increasing amounts of purified hemoglobin (to a maximum concentration of 19.3 mg/L) to aliquots of pooled serum samples. The hemoglobin significantly interfered with the dete...

A prominent clinical manifestation of sickle cell disease (SCD) is hemolytic anemia. Although complement activation can lead to intravascular hemolysis, its role in the hemolysis of SCD is not known. Because normal red blood cells induced to vesiculate by treatment with calcium and ionophore become sensitive to damage by activated complement and because sickle cells release microvesicles as the...

Thirty-seven per cent of 126 strains of Staphylococcus aureus, when tested on sheep blood-agar with sensitivity discs containing cephalothin, carbenicillin, oxacillin, penicillin, and cycloserine, produced rings of beta-hemolysis surrounding zones of inhibition of bacterial growth. Each strain capable of producing a ring of beta-hemolysis did so with at least two of the mentioned antibiotics. N...

The effect of peroxidative stress on tissue was studied by exposure of red blood cells (RBC) from patients with abetalipoproteinemia to minute amounts of H(2)O(2)in vitro. Red blood cells from untreated patients showed a marked sensitivity to H(2)O(2), as evidenced by hemolysis and lipid peroxidation (peroxidative hemolysis). The appearance of lipid peroxidation products in sensitive cells afte...

The effect of heparin upon the binding of the third component of complement (C3) to PNH red cells in vitro and their subsequent hemolysis is described. Heparin, in increasing concentrations, progressively inhibits membrane C3 fixation and hemolysis when the classic complement pathway is activated by anti-red cell antibodies. Heparin has a biphasic effect upon membrane C3 fixation and hemolysis ...

glucose 6-phosphate dehydrogenase (g6pd) deficiency is an enzyme deficiency of the red blood cells and the most important disease of hexose monophosphate pathway. the role of hemolysis in the pathophysiology of neonatal jaundice due to g6pd deficiency is in contencious. our aim is to study the role of hemolysis in neonatal jaundice associated with g6pd deficiency. this prospective descriptive s...

Pulmonary arterial hypertension (PAH) is suspected to be a strong mortality determinant of hemolytic disorders. However, direct contribution of acute intravascular hemolysis to fatal PAH has not been investigated. The roles of nitric oxide (NO) insufficiency and platelet activation in hemolysis-associated fatal PAH have been suspected but not been experimentally studied. We recently generated a...