The objective of this study was to develop a short-term culture system for buffalo spermatogonial stem cells (SSCs) in various culture conditions. Spermatogonial stem cells as well as sertoli cells were isolated from prepubertal buffalo calf testes and enriched to culture for two weeks in a serum-free medium. The serum-free medium was supplemented with various concentrations of serum (FBS) or f...

Here we proposed a new concept that human spermatogonial stem cells (SSCs) can transdifferentiate into hepatocytes in vivo. We first established liver injury model of mice by carbon tetrachloride to provide proper environment for human SSC transplantation. Liver mesenchymal cells were isolated from mice and identified phenotypically. Human SSC line was recombined with liver mesenchymal cells, a...

The theme of this fourth issue the 14th year magazine is “Women in Circuits.” This year’s guest editor, Qun Jane Gu, has invited four contributions from female leaders IEEE Solid-State Circuits Society (SSCS). Circuits” issues strive to promote diversity SSCS with vision inspire younger readers, improve advancement members, and increase visibility women within SSCS.

Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show th...

Most conventional features used in speaker authentication are based on estimation of spectral envelopes in one way or another, e.g., Mel-scale Filterbank Cepstrum Coefficients (MFCCs), Linear-scale Filterbank Cepstrum Coefficients (LFCCs) and Relative Spectral Perceptual Linear Prediction (RASTA-PLP). In this study, Spectral Subband Centroids (SSCs) are examined. These features are the centroid...

Student selected components (SSCs), at that time termed special study modules, were arguably the most innovative element in Tomorrow's Doctors (1993), the document from the General Medical Council that initiated the modernization of medical curricula in the UK. SSCs were proposed to make up one-third of the medical curriculum and provide students with choice, whilst allowing individual schools ...

BACKGROUND Knowledge about the identity and characteristics of spermatogonial stem cells (SSCs) in human is very limited. Here, Rhesus monkey was used as an animal model to investigate molecular and phenotypic characteristics of SSCs in the adult testes. METHODS A variety of immunohistological, molecular biological and functional assays were used to study different populations of SSCs in the ...

The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used...

Spermatogonial stem cells (SSCs) represent a unique population of germ cells with self-renewal potential. Although reactive oxygen species (ROS) are considered toxic to germ cells, we recently showed that moderate levels of ROS are required for SSC self-renewal and that Nox1 is involved in ROS generation. In this study, we showed that self-renewal factor treatment induces Nox3 to trigger SSC se...

identification of exogenous factors affecting spermatogonial stem cells (sscs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. the aim of this study was to investigate the effects of a gnrh analogue (alareline acetate) on sscs colonization in short-term co-culture with sertoli cells. five, three-month old holstein male calves were used to isolate spermatogo...