Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 microg Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 microg Mn/ml) was found to be toxic. The cell...

Background Apoptosis is believed to play a major role in the progressive weakening of the peri-infarct and remote zone myocardium after myocardial infarction (MI). Our laboratory previously developed an in vivo, MRI-detectable apoptosis probe. Annexin-V (ANX), which binds to cells undergoing apoptosis, was conjugated to superparamagnetic iron oxide (SPIO) nanoparticles, allowing for the non-inv...

Introduction: Apoptosis is a major consequence of ionizing radiation in proliferative tissues and quantification of the apoptotic cells could be helpful for noninvasive assessment and estimation of the radiation absorbed dose. Annexin V conjugated with super paramagnetic iron oxide nanoparticles (ANX-SPIO) is a biological probe for detection of apoptotic cells using magnetic resonance imaging. ...

Dendritic cells (DCs) generated in vitro to present tumour antigens have been injected in cancer patients to boost in vivo anti-tumour immune responses. This approach to cancer immunotherapy has had limited success. For anti-tumour therapy, delivery and subsequent migration of DCs to lymph nodes leading to effective stimulation of effector T cells is thought to be essential. The ability to non-...

PC-3 cancer cells. (A) [(AF750-BBN)]n-SPIO binding , and (B) blocked binding with BBN, red is AF750 and blue is Hoechst bluestained nucleus. (C) Cellular Prussian staining of [(AF750BBN)]n-SPIO. Fig. 2. In vivo fluorescence images and MRI in prostate cancer bearing SCID mice. Upper panel: images acquired after nanoparticles injection. Lower panel: after co-injection of the compound and unlabele...

MRI for in vivo stem cell tracking remains controversial. Here we tested the hypothesis that MRI can track the long-term fate of the superparamagnetic iron oxide (SPIO) nanoparticles labelled mesenchymal stem cells (MSCs) following intramyocardially injection in AMI rats. MSCs (1 × 10(6)) from male rats doubly labeled with SPIO and DAPI were injected 2 weeks after myocardial infarction. The con...

Background: The concept of targeted contrast agents can be readily adapted for use in applications such the specific and selective detection of cancer using MRI. Common implementations of this targeted approach have used targeting proteins specific to biomarkers overexpressed in certain cancers, conjugated to common contrast agents, such as superparamagnetic iron oxide (SPIOs) nanoparticles or ...

In vivo tracking of hematogenous macrophages has been a major challenge because these cells are key players in nerve injury and repair. We visualized the spatiotemporal course of macrophage infiltration after acute peripheral nerve injury in living rats by using superparamagnetic iron oxide (SPIO) particles and magnetic resonance imaging (MRI). A signal loss on MR images indicating iron accumul...

Short abstract (100 words) Detection of tumor metastases in the lymphatic system is essential for intra-operative staging of various malignancies. Pre-operative injection of clinically approved superparamagnetic iron oxide (SPIO) nanoparticles has been shown to improve MRI based nodal staging. The application of a clinical grade SPIO dispersion is verified by photoacoustically imaging ex vivo n...

Molecular magnetic resonance imaging (mMRI) has been paid more and more attention for early diagnosis of cancer. A sensitive and specific mMRI probe plays the most important role in this technique. In this study, superparamagnetic iron oxide (SPIO) nanoparticles and mAb G250 were conjugated as mMRI probe for the detection of clear cell renal cell carcinoma (ccRCC) using 3.0-Tesla MRI in vitro. ...