the freezing and thawing process not only is associated with serious damage to sperm such as damage to the plasma membrane and the acrosomal membrane but also changes the membrane permeability to some ions including calcium. also, the generation of oxygen free radicals is increased during the freezing-thawing process. the purpose of this study was to evaluate of the effects of trolox as an anti...

Caffeine is a well-known sperm motility stimulator, however, its effects on cooling-stored ram semen are unknown. The aim of the study was to examine the effect of caffeine on selected motility and viability indices of cooling-stored ram spermatozoa. Sperm ejaculates from 4 rams were diluted (1:3) in a Triladyl extender. Samples were stored for 96 h at 4-5 °C in two sets. In the first set used ...

background: the predominant phytoestrogen in soy and derived products is the isoflavone genistein. genistein has antioxidant properties. morphine is a main psychoactive chemical in opium that can increase the generation of free radicals and therefore it could adversely affects the spermatogenesis. objective: the main goal was to investigate whether the genistein could protect morphine adverse e...

Background: Low levels of reactive oxygen species (ROS) and calcium are necessary for sperm function. NADPH oxidase 5 (NOX5) is a membrane enzyme which produces ROS. This enzyme is dependent on calcium for its activity. We investigated the importance of NOX5 and an important calcium channel (CatSper) on sperm function.Methods: This laboratory in-vitro study was done in Shiraz, Iran, 2016. Norma...

fresh semen obtained from infertile men occasionally exhibits the total absence of motile sperm (100%asthenozoospermia). vitality tests may reveal that a proportion of the immotile sperm has functional membranes, which are considered viable. an easy test for the prior determination of sperm viability may assist in sperm selection for the microinjection of oocytes. the main objective of this stu...

Laboratories around the world have produced tens of thousands of mutant and transgenic zebrafish lines. As with mice, maintaining all of these valuable zebrafish genotypes is expensive, risky, and beyond the capacity of even the largest stock centers. Because reducing oxidative stress has become an important aspect of reducing the variability in mouse sperm cryopreservation, we examined whether...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

Fresh semen obtained from infertile men occasionally exhibits the total absence of motile sperm (100%asthenozoospermia). Vitality tests may reveal that a proportion of the immotile sperm has functional membranes, which are considered viable. An easy test for the prior determination of sperm viability may assist in sperm selection for the microinjection of oocytes. The main objective of this stu...

s:2011:"Background: Imidacloprid (IMD) is a systemic insecticide which acts as an insect neurotoxin and belongs to a class of chemicals called the neonicotinoids which act on the central nervous system of insects. According to previous studies, IMD is rated as "moderately toxic" on an acute oral basis to mammals and according to United States Environmental Protection Agency this compound is cla...

this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each...