BACKGROUND While (partial) recovery of spermatogenesis, observed by means of standard semen analysis, has been seen in testicular cancer patients after chemotherapy with cisplatin, sperm genomic integrity and its implication for the patient's fertility are poorly understood. METHODS Semen and serum from 22 patients treated for testicular cancer were analysed pre- and post-chemotherapy. Beside...

We investigated the motility and morphology of live sperm from barn swallows Hirundo rustica breeding in radioactively contaminated areas around Chernobyl and control areas in Ukraine in order to test the hypothesis that swimming behaviour and morphology of sperm was impaired by radioactive contamination. We obtained sperm samples from 98% of sampled birds, thus avoiding sampling bias due to th...

background: some evidences showed that the secretion of uterine tube vagina and follicular fluid (ff) affects x and y-chromosome populations. sperm selection with x or y chromosome can added to oocyte for gender desired. the isolation of x y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. the objective of this study was to find if the f...

OBJECTIVE To evaluate the spermatotoxicity of gold nanoparticles. DESIGN Experimental study. SETTING A university in Thailand. PATIENT(S) Single-donor fresh semen sample. INTERVENTION(S) A mixture of gold nanoparticle solution and semen was prepared and further analyzed. MAIN OUTCOME MEASURE(S) Spermatozoa appearance. RESULT(S) After mixing the semen with a gold nanoparticle solutio...

Background: The fertilizing sperm potential is directly related to its mitochondria activity evaluated by measuring the mitochondrial membrane potential (ΔΨm). The objectives of the present study were to investigate the vitality and mitochondrial membrane potential of sperm in infertile men by the and to assess the correlations between sperm parameters and the level of sperm mitochondrial membr...

BACKGROUND Global ubiquitination in human semen has been found to negatively correlate with standard semen parameters, indicating that ubiquitination can be considered a marker of poor semen quality. However, the inclusion of all semen components in the analysis may be misleading on the biological significance of ubiquitination of sperm cells. We have recently demonstrated the variable presence...

OBJECTIVE The objective of this study is to estimate the effects of Inclear, a feminine cleanser, on sperm motility. METHODS Semen samples were obtained from infertile male patients. Following liquefaction, the raw semen samples were diluted with Ham's F-10 nutrient mixture medium containing 0.4% human serum albumin solution at a ratio of 1:3. The semen samples were subsequently centrifuged t...

This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphomet...

OBJECTIVE This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS Semen analysis and a sperm DNA fragmen...

We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. ...