Boar spermatozoa were prepared for intracytoplasmic sperm injection (ICSI) by two different treatments to facilitate sperm chromatin decondensation and improve fertilisation rates after ICSI in pigs: spermatozoa were either frozen and thawed without cryoprotectants, or treated with progesterone. Morphological changes of the sperm heads after the treatments were examined and then the activation ...

Single-cell pulsed-field gel electrophoresis (SCPFGE) with dual electrode pairs was developed to detect the early stage of DNA fragmentation in human sperm. The motile sperm were purified by the commonly used density-gradient centrifugation technique and subsequent swim-up. The sperm were embedded in a thin film of agarose containing bovine trypsin (20 µg/mL) and were then lysed. Prior to SCPFG...

BACKGROUND Given the importance of timing of denudation and oocyte injection during assisted reproductive technology cycles, a study was undertaken of the impact of timing on the outcome of intracytoplasmic sperm injection in a cohort of patients from a private in vitro fertilization center. METHODS The study included patients who underwent fresh intracytoplasmic sperm injection-embryo transf...

OBJECTIVE The aim of this study was to evaluate the efficiency of the intracytoplasmic morphologically selected sperm injection (IMSI) technique compared with conventional ICSI and previous ICSI attempts in oligo-astheno-teratozoospermia (OAT) patients. METHODS The sperms were selected under high magnification (6,600×) and used to induce fertilization in previous ICSI patients by IMSI. These ...

BACKGROUND Intracytoplasmic morphologically selected sperm injection (IMSI) is still proposed and employed in the clinical practice to improve the reproductive outcome in infertile couples scheduled for conventional intracytoplasmic sperm injection (cICSI). The aim of the current randomized controlled trial (RCT) was to test the hypothesis that IMSI gives a better live birth delivery rate than ...

STUDY QUESTION Is the presence of nuclear vacuoles really a negative parameter? SUMMARY ANSWER As sperm vacuoles are associated with acrosomal and capacitation status, they appear to be a reflection of normal sperm physiology. WHAT IS KNOWN ALREADY The selection of sperm under a high magnification has been proposed as a strategy to increase the success rates of ICSI, through a better select...

objective: epididymo-orchitis (eo) potentially results in reduced fertility in up to 60% of affected patients. the anti-inflammatory effects of korean red ginseng (krg) and its ability to act as an immunoenhancer in parallel with the beneficial effects of this ancient herbal medicine on the reproductive systems of animals and humans led us to evaluate its protective effects against acute eo. ma...

The non-hormonal contraceptive, named RISUG (an acronym for Reversible Inhibition of Sperm under Guidance) has expected to provide a valuable addition to the current options of male contraception. The present study was conducted to determine the reproductive functional success, safety of vas occlusion by RISUG, and its reversal by dimethylsulphoxide (DMSO), followed by multigenerational (F1-F3)...

OBJECTIVE: The present study aimed to know the effect of paternal age together with Sperm DNA fragmentation on clinical and laboratory outcomes in donor oocyte intracytoplasmic sperm injection cycles. STUDY DESIGN: In this prospective cohort a total 229 patients undergoing their first cycles fresh blastocyst embryo transfer oocytes were included study. All categorized into four groups based mal...