نتایج جستجو برای: sperm function assay

تعداد نتایج: 1442869  

Journal: :Toxicological sciences : an official journal of the Society of Toxicology 2007
Eugenia Cordelli Anna Maria Fresegna Alessia D'Alessio Patrizia Eleuteri Marcello Spanò Francesca Pacchierotti Paola Villani

The increasing request of chemical safety assessment demands for the validation of alternative methods to reduce the resort to animal experimentation. Methods that evaluate reproductive toxicity are among those requiring the largest use of animals. Presently, no validated in vitro alternative exists for the assessment of reproductive toxicity. Mammalian sperm are sensitive targets of DNA-reacti...

Journal: :The Yale Journal of Biology and Medicine 1992
D. B. Brown M. Nagamani

Approximately one in six married couples find themselves involuntarily infertile. This ratio translates to between two and four million U.S. couples. Although numerous tests are available for diagnosing infertility problems, 5-10 percent of all couples who seek medical treatment are diagnosed with unexplained infertility. Several tests are presently available for diagnosing male infertility; ho...

Journal: :Journal of andrology 1990
A C Menge D A Ohl J Denil M K Korte L Keller M McCabe

Antisperm antibodies were assessed in the serum samples of 73 men unable to ejaculate naturally and on the sperm cells of 13 of these men. None of the serum samples were found to be positive by sperm agglutination or sperm immobilization methods and antibodies were detected by an immunobead assay on the sperm cells of one of the 13 men examined.

2018
Niloufar Hedayati Emami Farzaneh Mahmoudi Lafout Fahimeh Mohammadghasemi

Objectives Melatonin, an important hormone secreted by the epiphysis, is a powerful anti-oxidant with a high potential to neutralize medical toxins. The goal of this study was to demonstrate the beneficial effect of melatonin on epididymal sperm and reproductive parameters in mice treated with acetylsalicylic acid (ASA). Materials and Methods Male adult mice were divided into four treatment g...

2008
J. W. Byun S. H. Choo H. H. Kim Y. J. Kim Y. J. Hwang

MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored watersoluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studi...

2015
Carol Coughlan Helen Clarke Rachel Cutting Jane Saxton Sarah Waite William Ledger Tinchiu Li Allan A Pacey

Evidence is increasing that the integrity of sperm DNA may also be related to implantation failure and recurrent miscarriage (RM). To investigate this, the sperm DNA fragmentation in partners of 35 women with recurrent implantation failure (RIF) following in vitro fertilization, 16 women diagnosed with RM and seven recent fathers (control) were examined. Sperm were examined pre- and post-densit...

Journal: :The Journal of Cell Biology 1997
Jingdong Tian Hui Gong Gerald H. Thomsen William J. Lennarz

A quantitative assay was developed to study the interaction of Xenopus laevis sperm and eggs. Using this assay it was found that sperm bound in approximately equal numbers to the surface of both hemispheres of the unfertilized egg, but not to the surface of the fertilized egg. To understand the molecular basis of sperm binding to the egg vitelline envelope (VE), a competition assay was used and...

Journal: :Asian journal of andrology 2006
Petra Zrimsek Marjan Kosec Janez Kunc Janko Mrkun

AIM To assess that metabolic status of spermatozoa could provide a useful tool for evaluation of semen quality. METHODS The accuracy of the spectrophotometric application of the resazurin reduction assay was assessed using receiver operating characteristic (ROC) analysis. RESULTS Areas under ROC curves (AUC) for motile sperm concentration and sperm index (SI) (sperm concentration multiplied...

Journal: :Reproductive biology 2011
Natalie Moska Elaine Murray Penelope Wakefield Phillip Matson

The dark staining of human sperm heads by Diff Quik is significantly correlated with abnormal sperm head morphology (r=0.51, p<0.0001), but is not associated with changes in sperm chromatin detected by a sperm chromatin structure assay (SCSA; r=0.18, p>0.09). Whilst valuable in the assessment of head morphology, it is concluded that Diff Quik staining is not a useful substitute for the SCSA to ...

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