The freezing and thawing process not only is associated with serious damage to sperm such as damage to the plasma membrane and the acrosomal membrane but also changes the membrane permeability to some ions including calcium. Also, the generation of oxygen free radicals is increased during the freezing-thawing process. The purpose of this study was to evaluate of the effects of Trolox as an anti...

In the present study, motility, ultrastructure and fertilization capacity of fresh and cryopreserved striped bass spermatozoa were investigated in order to evaluate semen dilution ratio, freezing rate and cryomedia. Four dilution ratios (semen/cryomedia), and four freezing rates were evaluated on the basis of post-thaw sperm motility. The dilution ratio of 1:3 yielded the highest (P < 0.05) pos...

Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program | Filipp Georgijevic Savvulidi, Martin Ptacek, Anezka Malkova, Jakub Beranek, Ludek Stadnik Agricultural Journals

Background: Cryopreservation has adverse effect on human spermatozoa via exposure to physical and chemical stress that may lead to alter in reactive oxygen species (ROS) levels. During sperm preparation or cryopreservation, centrifugation as a physical stress may effect on sperm quality probably with stress oxidative production and membrane damage. Therefore the aim of this study was to evaluat...

The roe deer is a monoestrous species with very short rutting season. present work reports the most suitable period for collecting epididymal sperm and describes effect of two cooling rates on post-thaw quality sperm. Testes were collected 24–48 h after death. Samples flushed from epididymis subjected to either (1) dilution in Tris-citric acid-glucose-egg yolk-based medium glycerol, slow freezi...

OBJECTIVE Comparison of human sperm motility, morphology, and sperm membrane integrity using two cryoprotective media, Test-yolk buffer with glycerol and glycerol alone. METHODS Semen samples from 10 healthy donors were divided and frozen with either glycerol or a combination of glycerol and TEST-yolk buffer. Semen characteristics were evaluated before freezing and after thawing. Motility was...

Eriksson, B. 2000. Cryopresewation of boar semen. Studies on sperm viability in vitro and fertility. Doctoral thesis. ISSN 1401-6257. ISBN 91-576-5903-6 The world’s pig population is consistently being upgraded through the international trade of superior genetics. The two major systems that are used for this purpose are the transport of live animals and the export of frozen boar semen. The main...

Conventional sperm freezing methods perform best when freezing sperm samples containing at least hundreds of spermatozoa. In this severe male factor infertility case series, we examined the reproductive outcomes in 12 intracytoplasmic sperm injection cases where spermatozoa used were frozen in Cell Sleepers. Cell Sleepers are novel devices in which individual spermatozoa can be frozen in microd...

The study evaluated the protective effect of seminal plasma (SP) added to freezing extender against cryopreservation injuries to boar spermatozoa. Pooled sperm-rich fractions collected from 9 fertile boars were frozen in 0.5-mL straws after being extended in a conventional freezing extender either alone or supplemented with 5% of SPs (SP1-SP4) collected from the sperm-rich fractions (diluted 1:...

The present study was conducted to evaluate the in vivo fertility of frozen thawed boar semen. Twenty ejaculates collected from six mature boars were frozen in a programmable freezer. After freezing the semen was evaluated for different sperm quality parameters. Twenty five sows were inseminated artificially utilizing frozen thawed semen. The percentage of sperm motility, live sperm, live intac...