OBJECTIVE To evaluate the role of hyaluronic acid (HA) for sperm selection before intracytoplasmic sperm injection (ICSI). DESIGN Three prospective studies. SETTING Private assisted reproduction center in Italy. PATIENT(S) Study 1: 20 men. Study 2: 15 men. Study 3: 206 couples treated with ICSI on a limited number of oocytes per patient (1-3) in accordance with Italian IVF law. INTERVEN...

Sperm DNA fragmentation was analysed in 113 semen samples obtained from different rams over a period of one year. Semen samples were collected from: unvaccinated rams between January and June (Control group 1; CG1); vaccinated rams at least 70 days after vaccination between October and December (Control group 2; CG2); vaccinated rams 20 days after vaccination (Vaccinated group 1; VG1); and vacc...

BACKGROUND There are still concerns about the safety of intracytoplasmic sperm injection (ICSI) due to its brief clinical record and lack of animal testing. Testicular and epididymal sperm are now used routinely for ICSI in patients with obstructive azoospermia. The use of such immature sperm compounds fears, since little is known of their mitochondrial and nuclear DNA quality. METHODS A modi...

UNLABELLED The objectives of this cross-sectional observational study were: (i) to detect DNA damage and plasma membrane translocation of phosphatidylserine in purified sperm populations of high and low motility, and (ii) to analyse their relationship with the endogenous generation of reactive oxygen species. Ejaculates from infertile men were examined following gradient centrifugation. The mai...