The dark staining of human sperm heads by Diff Quik is significantly correlated with abnormal sperm head morphology (r=0.51, p<0.0001), but is not associated with changes in sperm chromatin detected by a sperm chromatin structure assay (SCSA; r=0.18, p>0.09). Whilst valuable in the assessment of head morphology, it is concluded that Diff Quik staining is not a useful substitute for the SCSA to ...

sperm is produced by the testis and mature in the epididymis. for having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. the effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. in recent years, the fe...

There is evidence to show that varicocele repair can improve conventional sperm parameters but the effects on sperm chromatin integrity have not been fully elucidated. We sought to examine the effects of varicocelectomy on sperm maturation, nuclear chromatin integrity and nuclear sulfhydryl groups. We conducted a prospective study of consecutive infertile men (n=29) that underwent a microsurgic...

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage chromatin. Therefore, quality was compared in samples following single-layer centrifugation (SLC) or and controls (extension only) two extenders, INRA82 INRA96. Ejaculates from six stallions were split among treatments: SLC, washing, controls, Sperm motility acrosome status evaluated at 0, 24, 4...

In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Background: Infertility is a complex medical problem. About 15% of couples are infertile, and male infertility being involved in roughly 50% of the cases. Among these, many cases are associated with a severe impairment of spermatogenesis. During the last stage of spermatogenesis (spermiogenesis), sperm chromatin endures complex modifications in which histones are lost and depositioned with tran...

Objective. To investigate sperm DNA fragmentation and sperm functional maturity in men from infertile couples (IC) and men with testicular germ cell tumor (TGCT). Materials and Methods. Semen samples were collected from 312 IC men and 23 men with TGCT before unilateral orchiectomy and oncological treatment. The sperm chromatin dispersion test was performed to determine DNA fragmentation index (...

Background: Globozoospermia is an uncommon disorder of sperm morphology associated with severe male infertility. Patients who contain both normal and round-headed sperm are called partial globozoospermic. It is possible that round-headed sperm may have an abnormal chromatin structure and /or DNA strand breaks. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated ...

Background: Following the expansion of human infertility, different procedures have been utilized for separation of normal sperm during ICSI (Intra cytoplasmic sperm injection). Hypo-osmotic swelling test (HOST) is one of the tests which have been introduced for assessment of integrity of sperm membrane when confronted with hypo osmotic condition. This test may also reflect intracellular organe...

Protamine, a small, strongly positively-charged protein, plays key role in achieving chromatin condensation inside sperm cells and is also involved the formulation of nanoparticles for gene therapy and...