introduction: wistar rats are an outbred strain of albino rats belonging to the species rattus norvegicus. histopathological studies on the testis was carried out to determine the effects of heat and lead on male fertility by evaluating some andrological parameters of the wistar rat such as morphology of spermatozoa, sperm count and motility. the main objective of this research was to compare t...

Successful sperm retrieval from ejaculates of nonmosaic Klinefelter's syndrome (KS) patients by using semen cytology examination was described in this report. The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients were proven to suffer from KS by chromosomal analysis using G-banding. Spermatozoa were obtained from ...

The selection of sperm cells possessing normal morphology and motility is crucial for many assisted reproductive technologies (ART), especially for intracytoplasmic sperm injection (ICSI), as sperm quality directly affects the probability of inducing healthy pregnancy. We present a novel platform for real-time quantitative analysis and selection of individual sperm cells without staining. Towar...

The yield of flow cytometric sorted Xand Y-chromosome-bearing sperm in a given time period is an important factor in the strategies used for fertilization and the production of sex-preselected offspring. This yield is dependent on the efficiency with which the modified flow cytometer/cell sorter analyzes the DNA of spermatozoa. The efficiency is directly related to the number of sperm with the ...

BACKGROUND Detection of apoptosis in sperm samples may help evaluate sperm quality. Recently, it has been suggested that in some ejaculated sperm populations, apoptosis is caspase dependent. The aim of this study was to investigate the presence of activated caspases and examine possible correlations with apoptosis and sperm parameters in semen samples prepared for IVF. METHODS To detect activ...

Background Sertoli cells located in seminiferous tubular basement membrane and surrounds different types of germ cell and they were only somatic cell type that directly contacts germ cells. These cells have Several role like support activities, nutrition of germ cells.During spermatogenesis, expression of SOX3 is necessary for differentiation of spermatogonia A. SOX3 is a transcription factor, ...

There are more than 80 million infertile couples around the world (1). It is estimated that about 40 million of infertile couples are currently seeking infertility treatments (2). Treatment of infertility ranges from simple advice on natural conception to complex and expensive interventions including Assisted Reproductive Technologies (ART) (3). Advanced infertility treatment methods are costly...

Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase in serum and sperm membrane chol, which ultimately altered membrane-coupled sperm specific functions: osmotic resistance, acrosomal reaction, and sperm capacitation in White New Zealand rabbits. These changes were also associated with a reduction in motility percentage and appearance of abnormal sperm morph...

The sperm/oocyte decision in the hermaphrodite germline of Caenorhabditis elegans provides a powerful model for the characterization of stem cell fate specification and differentiation. The germline sex determination program that governs gamete fate has been well studied, but direct mediators of cell-type-specific transcription are largely unknown. We report the identification of spe-44 as a cr...

OBJECTIVE To compare label-free interferometric phase microscopy (IPM) to label-free and label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This comparison helps in evaluating the potential of IPM for clinical sperm analysis without staining. DESIGN Comparison of imaging modalities. SETTING University laboratory. PATIENT(S) Sperm samples were obtained from heal...