Several methods for determination of the secondary structure of proteins by spectroscopic measurements are reviewed. Circular dichroism (CD) spectroscopy provides rapid determinations of protein secondary structure with dilute solutions and a way to rapidly assess conformational changes resulting from addition of ligands. Both CD and Raman spectroscopies are particularly useful for measurements...

Terahertz (THz) radiation lies between the microwave and infrared regions of the electromagnetic spectrum. THz radiation excites intermolecular interactions and is non-ionising making it a viable tool for medical imaging. This thesis describes the development and validation of spectroscopic methods for diagnosis of tissue pathologies at THz wavelengths. Theoretical techniques were developed to ...

Organophosphate pesticides are the ester forms of phosphoric acid usually considered as secure for agriculture uses due to their relatively fast degradation rates. Organophosphorus pesticides have been extensively used in the area of agriculture to manage insect or pests of a number of economically important crops. Organophosphate pesticides are well-known as the inhibitor of acetylcholinestera...

Qian, J. 2001. Mercury Species in Environmental Samples Studied by Spectroscopic Methods. Doctor k dissertation. ISSN 1401-6230, ISBN 91-576-6085-9. In this thesis, attention is focused on the determination, stability, reaction, and chemical association of mercury species in various types of environmental samples. Capillary gas chromatography (GC) was coupled to a number of systems for mercury ...

Statistical methods for helping characterise and make sense of spectroscopic data are of growing importance in biological research. The quantity of data describing biological objects and systems produced on a daily basis continues to increase, going hand-in-hand with developments in high-throughput techniques, assay multiplexing, minaturisation using nanofluidic devices, and, more commonly, wit...

In order to understand plant functioning, plant community composition, and terrestrial biogeochemistry, it is decisive to study standing root biomass, (fine) root dynamics, and interactions belowground. While most plant taxa can be identified by visual criteria aboveground, roots show less distinctive features. Furthermore, root systems of neighboring plants are rarely spatially segregated; thu...

From the aerial parts of Tanacetum balsamita L. (Compositae) a flavonol aglycon was iso­lated using chromatographic techniques. The structure of this compound was determined using spectroscopic methods (UV, H-NMR and MS) as 3',4',5,7-Tetrahydroxy flavonol (Quercetin).

the dimerization constants of safranine t have been determined by studying the dependence of its absorption spectra on the temperature in the range 30–70 ◦c at different total concentrations of safranine t (1.03×10−5, 1.44×10−5 and 1.73×10−5 m). the monomer–dimer equilibrium of safranine t has been determined by applying mcr-als method on the absorption spectra. the quantitative analysis of the...

This unit provides guidelines on how to use steady-state fluorescence spectroscopy for the quantification of protein-protein interactions. The fluorescence of a protein is characterized by its excitation and emission spectra, quantum yield, and anisotropy. These parameters can change upon interaction with another protein and can be used to measure the extent of complex formation. The source of ...