نتایج جستجو برای: species specific primers

تعداد نتایج: 1529553  

Journal: :Clinical and diagnostic laboratory immunology 2005
Sunlian Feng Karin Ku Emir Hodzic Edward Lorenzana Kim Freet Stephen W Barthold

Several species of helicobacter have been isolated from laboratory mice, including H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius, which appear to be the most common. The most widely used published method for molecular detection of these agents is PCR amplification of a conserved region of 16S rRNA, but differential speciation requires restriction enzyme digestion of the ...

Journal: :Frontiers in Ecology and Evolution 2022

Heosemys grandis , a species of Asian water turtle, that has wide range applications in the food and pharmaceutical industries. Since some processed products cannot be identified exclusively by morphological microscopic identification, reliable quick approach to guarantee authenticity is critical. Thus, we fostered an effective stable molecular identification system identify based on DNA mini-b...

Journal: :Applied and environmental microbiology 2004
Cinta Rachman Petia Kabadjova Rosica Valcheva Hervé Prévost Xavier Dousset

The genus Carnobacterium is currently divided into the following eight species: Carnobacterium piscicola, C. divergens, C. gallinarum, C. mobile, C. funditum, C. alterfunditum, C. inhibens, and C. viridans. An identification tool for the rapid differentiation of these eight Carnobacterium species was developed, based on the 16S-23S ribosomal DNA (rDNA) intergenic spacer region (ISR). PCR-restri...

Journal: :BMC Infectious Diseases 2003
Karine Ryffel Olivier Péter Eric Dayer Anne-Gabrielle Bretz Edmond Godfroid

BACKGROUND Although European Borrelia burgdorferi sensu lato isolates have been divided into five genospecies, specific tools for the serotype characterization of only three genospecies are available. Monoclonals antibodies (mAbs) H3TS, D6 and I17.3 identify B. burgdorferi sensu stricto (ss.), B. garinii and B. afzelii respectively, but no mAbs are available to identify B. valaisiana. In the sa...

Journal: :Applied and environmental microbiology 1997
P A Rochelle R De Leon M H Stewart R L Wolfe

Eight pairs of published PCR primers were evaluated for the specific detection of Cryptosporidium parvum and Giardia lamblia in water. Detection sensitivities ranged from 1 to 10 oocysts or cysts for purified preparations and 5 to 50 oocysts or cysts for seeded environmental water samples. Maximum sensitivity was achieved with two successive rounds of amplification and hybridization, with oligo...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1989
A M Wang M V Doyle D F Mark

A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which the same primers are used. The amount of mRNA is then quantitated by extrapolating against the standard curve generated with the internal stan...

Journal: :Applied and environmental microbiology 1999
T Matsuki K Watanabe R Tanaka M Fukuda H Oyaizu

In order to clarify the distribution of bifidobacterial species in the human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from fecal samples obtained from 48 healthy adults and 27 breast-fed infants. To cover all of the bifidobacterial species that have been isolated from and identified in the human intestinal tract, specie...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید