Pogonatherum crinitum is a promising lead (Pb) hyperaccumulator; however, the effects of Pb contamination on P. rhizosphere soil enzymatic activities and microbial composition remain largely unexplored. Thus, an indoor experiment was conducted by cultivating seedlings exposing them to four concentrations (0, 1,000, 2000 3000 mg/kg Pb). Protease, urease, acid phosphatase invertase were determine...

Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium Yersinia pseudotuberculosis (Yptb). However, whether RovM regulates the urease activity in Yptb was still unknown. In this study, by using qualitative and...

Water-soluble compounds (WS) obtained from the pyrolysis of three lignocellulosic biomasses (larch, poplar, and switchgrass) were tested as potential inhibitors enzyme urease. Thanks to presence an array phenolic like catechol, methoxy/hydroxy phenols, acids, aldehydes, all WS samples at a catechol concentration 30 μM inhibited activity jack bean urease (JBU) by 60%–70% 80% that naturally prese...

Field experiments are described involving nitrogen fertilizer in wheat field to research its effect on microbial quantity and enzyme activity. The results showed that 250 kg N hm applied in wheat field (1) can increase bacteria, fungi, actinomycetes quantity by 39.9%, 56.7% and 70.5% compare to 0 kg N hm. (2) Similarly, the activities of soil urease, catalase and FDA expressed 60.8%, 18.3% and ...

Forestry works using chainsaws result in up to 7 million liters of various mineral oils being soaked annually into forest soils. These substances, containing a complex mixture of polycyclic aromatic hydrocarbons (PAHs), are highly toxic. The aim of the study was to determine the effect of oil contamination with PAHs on the physical and biological properties of forest soils. The study area was l...

With 110-d incubation experiment in laboratory, the responses of microbial quantity, soil enzymatic activity, and bacterial community structure to different amounts of diesel fuel amendments were studied to reveal whether certain biological and biochemical characteristics could serve as reliable indicators of petroleum hydrocarbon contamination in meadow-brown soil, and use these indicators to ...

Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, d...

The incorporation of biosolids to soil is a strategy aiming at the re-location of these materials in the environment with a useful end: soil fertilization. In this work, the response of two Argiudoll soils (one with more than 100 years of agriculture and the other, a virgin one) to biosolid incorporation was studied under laboratory conditions. To measure this response, soil enzymatic biodescri...

The relationship between plant species coexistence and soil microbial communities under heavy metal pollution has attracted much attention in ecology. However, whether plant species coexistence could offset the impacts of heavy metal combined pollution on soil microbial community structure and soil enzymes activities is not well studied. The modified ecological dose model and PCR-RAPD method we...