The preparation and properties of an acetate activating enzyme’ from animal tissues have been described by Beinert et al. (1). In that work it had been noted that the sodium ion is a potent inhibitor of acetyl CoA formation in the acetate-ATP reaction. Nachmansohn and John (2) reported stimulation of the choline-acetylating system by potassium ion, while Korey et al. (3) reported the inhibition...

An experimental approach for the production of catalyst-free hyperpolarised ethanol solution in water via heterogeneous hydrogenation of vinyl acetate with parahydrogen and the subsequent hydrolysis of ethyl acetate was demonstrated. For an efficient hydrogenation, liquid vinyl acetate was transferred to the gas phase by parahydrogen bubbling and almost completely converted to ethyl acetate wit...

An inducible acetylesterase (EC 3.1.1.6) that hydrolyzes ethyl acetate, an intermediate in the degradation of 2-butanone by Nocardia strain LSU-169, was purified. The polypeptide molecular weight as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 39,500, and the enzyme molecular weight determined by sucrose density gradient centrifugation was 84,000. The purified enz...