A triacylglycerol lipase, presumably the first enzyme involved in the mobilization of lipid from the insect fat body, has been purified to homogeneity from the fat body of Manduca sexta. The purification procedure involved polyethyleneglycol precipitation, and chromatography on DEAE-cellulose, phenyl-Sepharose, Q-Sepharose and hydroxylapatite. The final product, a protein with an M(r) = 76,000 ...

A previous report from this laboratory (van Golde, L.M.G., Schulman, H., and Kennedy, E.P. (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 1368-1372) described the discovery in Escherichia coli of a novel class of oligosaccharides, containing glucose as the sole sugar, substituted with glycerophosphate units derived from membrane phospholipids, and with succinic acid in O-ester linkage. These membrane...

Glycerophospholipids with two, non-equivalent fatty acyl chains can adopt one of two isomeric forms depending on the relative position of substitutions on the glycerol backbone. These so-called sn-positional isomers can have distinct biophysical and biochemical behaviors making it desirable to uniquely assign their regiochemistries. Unambiguous assignment of such similar molecular structures in...

The attempt to secure conclusive, spectroscopic evidence for the GRB/SN connection has been a central theme in most GRB observing time proposals since the discovery of the very unusual GRB 980425 associated with the peculiar type Ib/c SN 1998bw. GRB 030329 provided this evidence to everybody’s satisfaction. In this contribution we show the results of a spectroscopic campaign of the supernova as...

In the present study, we propose a continuous assay for the screening of sn-2 lipases by using triacylglycerols (TAGs) from Aleurites fordii seed (tung oil) and a synthetic TAG containing the α-eleostearic acid at the sn-2 position and the oleic acid (OA) at the sn-1 and sn-3 positions [1,3-O-dioleoyl-2-O-α-eleostearoyl-sn-glycerol (sn-OEO)]. Each TAG was coated into a microplate well, and the ...

The alterations induced on the pool sizes of five phospholipid metabolites, glycerol 3-phosphorycholine, glycerol 3-phosphorylethanolamine, phosphorylcholine, sn-glycerol 3-phosphate, and choline were studied by nuclear magnetic resonance (NMR) spectroscopy in murine tumors injected with recombinant murine tumor necrosis factor (TNF). Solid tumors were obtained by s.c. injection of either Frien...

The fast atom bombardment spectra of three phospholipids containing a secondary, tertiary, and quaternary nitrogenous base attached to a 1,2-dipalmitoyl-sn-glycero-3-phosphate skeleton were shown to each contain an ion derived from the intact nitrogenous base. The collisional activation mass analyzed ion kinetic energy spectroscopy (CA MIKES) spectrum produced from each of these ions was shown ...

The in situ reaction of the tripodal anion [EtAl((Me)Py)3](-) ((Me)Py = 6-Me-2-py) with SnCl2 gives the Janus-head ligand ((Me)Py)3Sn-Sn((Me)Py)3, containing a Sn-Sn bond, through a novel, one-pot reaction involving a combination of pyridyl transfer and metal-oxidation.

Q 2 evolution of structure functions in the nucleon and nuclei Abstract Q 2 evolution of structure functions in the nucleon and nuclei is investigated by using usual DGLAP equations and parton-recombination equations. Calculated results for proton's F 2 and for the ratio F Ca 2 /F D 2 are compared with various experimental data. Furthermore, we study nuclear dependence of Q 2 evolution in tin a...