Skin stem cells are very important in cosmetics, pharmacological and regenerative medicine and burn cases. Foreskin samples surgically removed after circumcision from boys below 7 years were collected and primary epidermal cells were prepared by enzymatic and mechanical tituration method. Selecting CD133 (prominin-1) multipotent stem cell marker, enriched stem cells were analyzed by MACS using ...

The mammalian skin is the largest organ of the body and its outermost layer, the epidermis, undergoes dynamic lifetime renewal through the activity of somatic stem cell populations. The EGFR-Ras-Raf pathway has a well-described role in skin development and tumor formation. While research mainly focuses on its role in cutaneous tumor initiation and maintenance, much less is known about Ras signa...

purpose : our aim was to isolate mesenchymal stem cells (mscs) from mouse umbilical cord and culture them with vitreous body to investigate their capacity to differentiate into lens fiber cells, crystallins are expressed. methods : wharton's jelly is the appropriate source of mscs. after isolation, the cells were cultured and alkaline phosphatase detection kit was used for staining undiffe...

background: mesenchymal stem cells (mscs) are undifferentiated cells that can differentiate and divide to other cell types. transplantation of these cells to the different organs is used for curing various diseases. objective: the aim of this research was whether mscs transplantation could treat the sterile testes. materials and methods: in this experimental study, donor mscs were isolated from...

Back ground: Basal cell carcinomas and follicular tumors are relatively common skin neoplasms both in veterinary and human medicine. Currently, it is believed that stem cells are important for skin tumor development. Hypothesis/objective: the objective of the study was to characterize CD34 expression in canine follicular tumors and basal cell carcinoma in order to investigate the possible role ...

Adult epidermal stem cells renew the epithelial compartment of the skin throughout life and are the most accessible of all adult stem cells. Most importantly, epidermal stem cells can be efficiently cultivated and transplanted, a significant advantage for cell and gene therapy. Recent work has pointed to the hair follicle as the main repository of multipotent stem cells in skin. Hair follicles,...

ex vivo expansion of human umbilical cord blood cells (hucbc) is explored by several investigators to enhance the repopulating potential of hucbc. the proliferation and expansion of human hematopoietic stem cells (hsc) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding hsc for patient transplantation. using primary human mesenchymal stem cells...

The location of follicular and epidermal stem cells in mammalian skin is a crucial issue in cutaneous biology. We demonstrate that hair follicular stem cells, located in the bulge region, can give rise to several cell types of the hair follicle as well as upper follicular cells. Moreover, we devised a double-label technique to show that upper follicular keratinocytes emigrate into the epidermis...

BACKGROUND The combination of cells with platelet-rich plasma (PRP) may fulfill tendon deficits and help overcome the limited ability of tendons to heal. PURPOSE To examine the suitability of 3 human cell types in combination with PRP and the potential impact of the tenocyte-conditioned media (CM) to enhance tendon healing. STUDY DESIGN Controlled laboratory study. METHODS Tenocytes, bone...