A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...

in the present study, random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers were used to estimate genetic diversity and relationships among 35 cluster bean [cyamopsis tetragonoloba (l.) taub] genotypes. for rapd analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. the amplified pro...

In Coffea arabica (arabica coffee), the phenotypic as well as genetic variability has been found low because of the narrow genetic basis and self fertile nature of the species. Because of high similarity in phenotypic appearance among the majority of arabica collections, selection of parental lines for inter-varietals hybridization and identification of resultant hybrids at an early stage of pl...

The relationship among six Jordanian almond landraces was studied using morphological traits and RAPD analyses. Across studied, nut shape, kernel size, length, shell length That showed a high level of variation (CV>30%), while width, internodes width comparatively low values (CV<20%). Principal component analysis that the first five components explained all investigated. Kernel were predo...

Three wild populations of Clarias gariepinus from Esaodo (River Osun), Owena (River Owena), and Agbabu (River Oluwa), and three farmed populations, viz: Akure, Ilesa and Ado-Ekiti, in Southwest Nigeria, were analysed for their genetic differences using Random amplified polymorphic DNA (RAPD) analysis. Live specimens comprising 40 individuals (680 ± 3.28 g) from each location were collected and ...

We evaluated the ability of randomly amplified polymorphic DNA (RAPD) to type citrinin-producing Penicillium citrinum (P. citrinum) strains recovered from the forest's air in northern Iran. A total of 12 P. citrinum strains (P1-P12) were characterised by citrinin production and random amplification of polymorphic DNA (RAPD) technique. All the strains produced citrinin with levels ranging from 1...

Estimates of genetic diversity can be based on different types of data. The aim of this research were to study genetic diversity among Croatian wheat cultivars by random amplified polymorphic DNA (RAPD) markers, morphological traits and pedigree records; to analyse differences between wheat cultivars from two breeding centres; and to evaluate usability of RAPD markers for estimation of genetic ...