نتایج جستجو برای: sirna targeting cassette

تعداد نتایج: 175780  

2018
Xiaolin Yu Sharad Ghamande Haitao Liu Lu Xue Shuhua Zhao Wenxi Tan Lijing Zhao Shou-Ching Tang Daqing Wu Hasan Korkaya Nita J. Maihle Hong Yan Liu

HER family members are interdependent and functionally compensatory. Simultaneously targeting EGFR/HER2/HER3 by antibody combinations has demonstrated superior treatment efficacy over targeting one HER receptor. However, antibody combinations have their limitations, with high immunogenicity and high cost. In this study, we have developed a three-in-one nucleic acid aptamer-small interfering RNA...

2012
Miao Zhong Shafiq A. Khan

Celland receptor-specific regulation of cell migration by Gi/oa-proteins remains unknown in prostate cancer cells. In the present study, oxytocin (OXT) receptor was detected at the protein level in total cell lysates from C81 (an androgen-independent subline of LNCaP), DU145 and PC3 prostate cancer cells, but not in immortalized normal prostate luminal epithelial cells (RWPE1), and OXT-induced ...

2013
Kouji Izumi Lei-Ya Fang Atsushi Mizokami Mikio Namiki Lei Li Wen-Jye Lin Chawnshang Chang

Increased CCL2 expression in prostate cancer (PCa) cells enhanced metastasis via macrophage recruitment. However, its linkage to androgen receptor (AR)-mediated PCa progression remains unclear. Here, we identified a previously unrecognized regulation: targeting AR with siRNA in PCa cells increased macrophage recruitment via CCL2 up-regulation, which might then result in enhancing PCa invasivene...

2013
Pei-Chi Wei Zi-Fu Wang Wen-Ting Lo Mei-I Su Jin-Yuh Shew Ta-Chau Chang Wen-Hwa Lee

We reported that non-targeting siRNA (NT-siRNA) stress induces non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) expression to cooperate with exoribonuclease XRN2 for releasing the stress [Wei,P.C., Lo,W.T., Su,M.I., Shew,J.Y. and Lee,W.H. (2011) Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nu...

Amoabediny, Ghasem , Haghiralsadat, Bibi Fatemeh , Morteza-Ratki, Farzane , Naderinezhad, Samira , Zandieh Doulabi, Behrouz ,

Introdution: Cationic liposomes have been presented for gene delivery as an alternative vector instead of viral vectors. A major challenge associated with siRNA delivery is the instability of liposomes, which is still a serious problem. The aim of this study was to provide an appropriate formulation to overcome this instability. Methods: In the present study (Scientific-Fundamental, Experiment...

2016
Cynthia Yu-Wai-Man Aristides D. Tagalakis Maria D. Manunta Stephen L. Hart Peng T. Khaw

There is increasing evidence that the Myocardin-related transcription factor/Serum response factor (MRTF/SRF) pathway plays a key role in fibroblast activation and that knocking down MRTF can lead to reduced scarring and fibrosis. Here, we have developed a receptor-targeted liposome-peptide-siRNA nanoparticle as a non-viral delivery system for MRTF-B siRNA in conjunctival fibrosis. Using 50 nM ...

Journal: :Cancer discovery 2013
Masato Nishimura Eun-Jung Jung Maitri Y Shah Chunhua Lu Riccardo Spizzo Masayoshi Shimizu Hee Dong Han Cristina Ivan Simona Rossi Xinna Zhang Milena S Nicoloso Sherry Y Wu Maria Ines Almeida Justin Bottsford-Miller Chad V Pecot Behrouz Zand Koji Matsuo Mian M Shahzad Nicholas B Jennings Cristian Rodriguez-Aguayo Gabriel Lopez-Berestein Anil K Sood George A Calin

UNLABELLED Development of improved RNA interference-based strategies is of utmost clinical importance. Although siRNA-mediated silencing of EphA2, an ovarian cancer oncogene, results in reduction of tumor growth, we present evidence that additional inhibition of EphA2 by a microRNA (miRNA) further "boosts" its antitumor effects. We identified miR-520d-3p as a tumor suppressor upstream of EphA2,...

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