Many genome-wide datasets are routinely generated to study different aspects of biological systems, but integrating them to obtain a coherent view of the underlying biology remains a challenge. We propose simultaneous clustering of multiple networks as a framework to integrate large-scale datasets on the interactions among and activities of cellular components. Specifically, we develop an algor...

We have designed AdenoQuick, a fast and versatile method to construct first-generation adenoviral vectors that contain one or two transgenes in the E1 and/or the E3 region. The method is based on the reconstitution of the entire genome of the desired recombinant virus in E. coli and the subsequent transfection of the DNA in a helper cell line. Since the construction of large adenoviral plasmids...

BACKGROUND The optimization of industrial bioethanol production will depend on the rational design and manipulation of industrial strains to improve their robustness against the many stress factors affecting their performance during very high gravity (VHG) or lignocellulosic fermentations. In this study, a set of Saccharomyces cerevisiae genes found, through genome-wide screenings, to confer re...

AIMS This study was designed to demonstrate simultaneous increases in proliferation and apoptosis of vascular smooth muscle cells (VSMCs) leading to accelerated vein graft remodeling and to explore the underlying mechanisms. METHODS Vein grafts were performed in non-diabetic and diabetic mice. The cultured quiescent VSMCs were subjected to mechanical stretch stress (SS) and/or advanced glycos...

Abstract Background Clinical applications of RNA interference for cancer treatment and immune therapy require the development simultaneous imaging systems microRNA. This research was performed to fabricate miRNA34a-loaded magnetic nanoparticles investigate its anticancer effects against triple-negative breast (TNBC) in mice model. Results Using two types polymers improve their water dispersibil...

introduction: in order to study the alterations of beta 1 and 2 integrins mrna level in rat lumbar spinal cord following the induction of chronic pain and its effect on the development of tolerance to morphine analgesia, we examined the level of expression of these genes in the presence of chronic pain, which is an inhibitor of morphine tolerance. we used induction of chronic pain alone and in ...

We present a novel approach to word reordering which successfully integrates syntactic structural knowledge with phrase-based SMT. This is done by constructing a lattice of alternatives based on automatically learned probabilistic syntactic rules. In decoding, the alternatives are scored based on the output word order, not the order of the input. Unlike previous approaches, this makes it possib...

........................................................................................................................................ 2 Zusammenfassung ........................................................................................................................ 3 Acknowledgement ..........................................................................................................