نتایج جستجو برای: simple pcr
تعداد نتایج: 627586 فیلتر نتایج به سال:
Simple and Accurate PCR-Based System for Typing Vacuolating Cytotoxin Alleles of Helicobacter pylori
A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods
Background and purpose: Salmonella is a group of Enterobacterias that cause infectious diseases in human and animals. Typhoid, bacteremia, enterocolitis and salmonellosis which are caused by this bacterium are considered major health concerns in developing countries, such as Iran. Quick, accurate, reliable and early diagnosis is vital to prevent Salmonella outbreak. Materials and methods: Ther...
Background: Yersinia pestis and Francisella tularensis cause plague and tularemia, which are known as diseases of the newborn and elderly, respectively. Immunological and culture-based detection methods of these bacteria are time-consuming, costly, complicated and require advanced equipment. We aimed to design and synthesize a gene structure as positive control for molecular detection of these ...
We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5' end. Treatment of the PCR products with a commercial deoxyuridine-excision reagent generates long 3' overhangs designed to specifically complement each other. The combin...
Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR d...
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