The worldwide dissemination of extended-spectrum-β-lactamase (ESBL)- and carbapenemase-producing Enterobacteriaceae is a major concern in both hospital and community settings. Rapid identification of these resistant pathogens and the genetic determinants they possess is needed to assist in clinical practice and epidemiological studies. A collection of Escherichia coli, Klebsiella pneumoniae, Kl...

A new description of the exact completion Cex/reg of a regular category C is given, using a certain topos Shv(C) of sheaves on C; the exact completion is then constructed as the closure of C in Shv(C) under finite limits and coequalizers of equivalence relations. An infinitary generalization is proved, and the classical description of the exact completion is derived.

A new description of the exact completion C ex/reg of a regular category C is given, using a certain topos Shv(C) of sheaves on C; the exact completion is then constructed as the closure of C in Shv(C) under nite limits and coequalizers of equivalence relations. An innnitary generalization is proved, and the classical description of the exact completion is derived.

To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producin...

One hundred four Enterobacter isolates were tested by standard CLSI disk diffusion methods for detecting extended-spectrum beta-lactamases (ESBLs) and with cefepime-clavulanate disk combinations. SHV-12 was produced by 8.7% of isolates. The cefepime-clavulanate combination provided 88% sensitivity and 91% specificity for the detection of SHV-12 ESBL.

A beta-lactamase with an M(r) of 29,000 and a pI of 7.6 was partially purified from a clinical isolate of Shigella dysenteriae. The bla gene encoded the SHV-11 enzyme carrying the substitution Leu-->Gln at position 35 and was linked to a strong promoter. This variant, unlike the prototype SHV-1 enzyme, hydrolyzed oxacillin, cloxacillin, and oxyiminocephalosporins such as cefotaxime.

BACKGROUND & OBJECTIVE Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in gram-negative isolates. ESBLs are mainly coded for by three important genes, namely bla(TEM), bla(SHV) and bla(CTX-M). In this study we used a multiplex PCR as a rapid method to identify two common genes (bla(CTX-M) & bla(SHV)) responsible for extended spectrum beta lactama...

The preferred embodiment of dual linear polarization technology on the WSR-88D weather radars is the mode whereby horizontal (H) and vertical (V) polarizations are transmitted and received simultaneously (Doviak et al. 2000). This mode is sometimes referred to as “hybrid” (Wang et al. 2006); to shorten notation we label it SHV (Simultaneous Horizontal and Vertical). The USA National Weather Ser...