introduction: infections by multidrug resistant mycobacterium tuberculosis (mdr-tb) is a major public health challenge. secretion of proteins by m. tuberculosis plays an important role in the pathogenesis of the bacterium. we compared the protein profiles of susceptible m. tuberculosis and mdr-tb isolates using proteomic analyses, namely two dimensional gel electrophoresis (2de) and mass spectr...

1 Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA 2Department of Academic Rheumatology, King’s College, London SE1 1UL, UK 3Department of Cellular and Developmental Biology (STEMBIO), University of Palermo, Viale delle Scienze, 90128 Palermo, Italy 4Department of Neurology and Neuromuscular Reference Center, Ghent University Hospita...

The Heat Is on : Heat-Shock Proteins and Atherosclerosis Print ISSN: 0009-7322. Online ISSN: 1524-4539 Copyright © 2006 American Heart Association, Inc. All rights reserved. is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Circulation doi: 10.1161/CIRCULATIONAHA.106.647875 2006;114:870-872 Circulation. http://circ.ahajournals.org/content/114/9/870 World W...

Heat shock proteins are highly conserved proteins present in organisms ranging from bacteria to man. They are both dominant microbial immunogens and among the first proteins produced during mammalian embryo development. Since bacterial and human heat shock proteins share a high degree of amino acid sequence homology, it has been suggested that sensitization to bacterial heat shock proteins duri...

We have measured the effect of heat shock on three mycoplasmas (Acholeplasma laidlawii K2 and JA1 and Mycoplasma capricolum Kid) and demonstrated the induction of mycoplasma heat shock proteins under these conditions. Increased synthesis of at least 5 heat shock proteins in A. laidlawii K2, 11 heat shock proteins in A. laidlawii JA1, and 7 heat shock proteins in M. capricolum was observed by el...

Cells may sense heat shock via the accumulation of thermally misfolded proteins. To explore this possibility, we determined the effect of protein misfolding on gene expression in the absence of temperature changes. The imino acid analog azetidine-2-carboxylic acid (AZC) is incorporated into protein competitively with proline and causes reduced thermal stability or misfolding. We found that addi...

The induction of acid-shock proteins and the degree of acid resistance conferred on Clostridium perfringens by acid shock, and the kinetics of this resistance were determined. A sublethal acid shock at pH 4.5 for 20 min increased the acid tolerance of cells at least fifteenfold. The acquired tolerance was maintained for 3 h after acid treatment. The response of the microorganism to acid shock w...

Cultured tobacco cells (Nicotiana tabacum L. var Wisconsin-38) developed tolerance to otherwise nonpermissive 54 degrees C treatment when heat-shocked at 38 degrees C (2 h) but not at 42 degrees C. Heat-shocked cells (38 degrees C) exhibited little normal growth when the 54 degrees C stress came immediately after heat shock and normal growth when 54 degrees C stress was administered 8 hours aft...

The pattern of protein synthesis changes rapidly and dramatically when the growth temperature of soybean seedling tissue is increased from 28 degrees C (normal) to about 40 degrees C (heat shock). The synthesis of normal proteins is greatly decreased and a new set of proteins, "heat shock proteins," is induced. The heat shock proteins of soybean consist of 10 new bands on one-dimensional NaDodS...