^cience Congress, held at Lahore in January 19^7.) Radius and Gentlemen, _ j t consider it my duty in the first instance to express j my grateful appreciation of the great honour that you ?ve done me in electing me to occupy the Presidential l chair. Not only do I look upon it as a personal honour, ? to me it is an appreciation of the institution to ^nich it is my privilege to belong, and which...

We have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 5' and 3' ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore-forming region of the protein. Three different splice variants were found within the P region, two of wh...

Kv4.2 is a voltage-gated potassium channel that is critical in controlling the excitability of myocytes and neurons. Processes that influence trafficking and surface distribution patterns of Kv4.2 will affect its ability to contribute to cellular functions. The scaffolding/clustering protein PSD-95 regulates trafficking and distribution of several receptors and Shaker family Kv channels. We the...

Though current expert system technology has become a major success, e x i s t i n g expert systems o f ten f a l l short of human exper t ise In many ways. One Important area is in the use of more bas ic , deep knowledge as an enhancement to the shal low, surface knowledge commonly employed. The In tegra t ed Diagnost ic Model attempts to e x p l o i t the use of both types of knowledge by f i ...

The Kv2.1 voltage-activated K+ channel, a Shab-related K+ channel isolated from rat brain, is insensitive to previously identified peptide inhibitors. We have isolated two peptides from the venom of a Chilean tarantula, G. spatulata, that inhibit the Kv2.1 K+ channel. The two peptides, hanatoxin1 (HaTx1) and hanatoxin2 (HaTx2) are unrelated in primary sequence to other K+ channel inhibitors. Th...

Expression of appropriate ion channels is essential to allow developing neurons to form functional networks. Our previous studies have identified LIM-homeodomain (HD) transcription factors (TFs), expressed by developing neurons, that are specifically able to regulate ion channel gene expression. In this study, we use the technique of DNA adenine methyltransferase identification (DamID) to ident...

Voltage-gated potassium channels are critical elements in providing functional diversity in nervous systems. The diversity of voltage-gated K+ channels in modern triploblastic metazoans (such as mollusks, arthropods and vertebrates) is provided primarily by four gene subfamilies (Shaker, Shal, Shab, and Shaw), but there has been no data from the ancient diploblastic metazoans until now. Diplobl...

Dopamine (DA) modifies the motor pattern generated by the pyloric network in the stomatogastric ganglion (STG) of the spiny lobster, Panulirus interruptus, by directly acting on each of the circuit neurons. The 14 pyloric neurons fall into six cell types, and DA actions are cell type specific. The transient potassium current mediated by shal channels (I(A)) is a common target of DA modulation i...

To identify genes that promote facial skin youthfulness (SY), a genome-wide association study on an Ashkenazi Jewish discovery group (n=428) was performed using Affymetrix 6.0 Single-Nucleotide Polymorphism (SNP) Array. After SNP quality controls, 901,470 SNPs remained for analysis. The eigenstrat method showed no stratification. Cases and controls were identified by global facial skin aging se...

Novel Multi-dimensional Upwind Constrained Transport (MUCT) schemes on un-structured triangular grids are described. Constrained Transport (CT) discretizations conserve the divergence-free nature of divergence-free vector fields on the discrete level. Multi-dimensional Upwind (MU) schemes generalize the concept of dimensionally split upwind schemes for hyperbolic systems to truly multidimension...