Cells are sensitive to mechanical cues from their environment and at the same time generate and transmit forces to their surroundings. To test quantitatively forces generated by cells not attached to a substrate, we used a dual optical trap to suspend 3T3 fibroblasts between two fibronectin-coated beads. In this simple geometry, we measured both the cells' elastic properties and the force fluct...

compensatory growth response and body composition of male sailfin molly, poecilia latipinna subjected to short-term starvation and subsequent feeding were studied for 54 days. four feeding schedules were used in this study: c, control (were fed to apparent satiation throughout the experiment); t1, treatment 1 (3 days starvation and 6 days refeeding); t2, treatment 2 (6 days starvation and 12 da...

fish may experience periods of food deprivation or starvation in nature (such as seasonal migration and reproduction) and under culture conditions (unpredictable stress such as transporting and sorting). with respect to vital role of starvation periods on body composition, the current study was carried out in autumn 2010 at international sturgeon research institute. after adaptation for one mon...

The tumor suppressor p53 is widely known for its ability to induce cell cycle arrest or cell death, therefore preventing neoplastic progression. Previous studies have demonstrated novel roles for p53 in the regulation of autophagy and senescence. p53 can not only exert cell cycle‑arresting and senescence‑promoting or suppressing functions, but can also induce autophagic flux, particularly under ...

The regulation of protein synthesis was studied in KRC-7 cells (rat hepatoma) grown in complete medium, during serum starvation, and mitogen activation. Upon serum starvation, the cells lost almost completely p67 mRNA, p67 protein, and protein synthesis activity. After phorbol 12-myristate 13-acetate addition, the same serum-starved cells regained p67 mRNA, p67 protein, and protein synthesis ac...

The epidermal growth factor receptor (EGFR) is upregulated in numerous human cancers. Inhibition of EGFR signaling induces autophagy in tumor cells. Here, we report an unanticipated role for the inactive EGFR in autophagy initiation. Inactive EGFR interacts with the oncoprotein LAPTM4B that is required for the endosomal accumulation of EGFR upon serum starvation. Inactive EGFR and LAPTM4B stabi...

Some viral infections are reported to influence the susceptibility of peripheral blood mononuclear cells (PBMC) to apoptosis, which is related to disease progression. The current study was designed to monitor apoptosis in separated PBMC subsets, CD4+ and CD8+ T lymphocytes, and CD14+ monocytes under apoptotic stimuli in patients with chronic hepatitis C. Apoptosis was induced by serum starvatio...

Elemene, a compound found in an herb used in traditional Chinese medicine, has shown promising anticancer effects against a broad spectrum of tumors. In an in vivo experiment, we found that apatinib, a tyrosine kinase inhibitor that selectively inhibits VEGFR2, combined with elemene injection (Ele) for the treatment of H22 solid tumor in mice resulted in worse effectiveness than apatinib alone....

All subjects were obese and were admitted to hospital for therapeutic starvation. The majority were female, insufficient males being included to allow a between-sex comparison. After I to 2 days on a normal ward diet when base-line values were obtained, subjects underwent 14 days of total starvation (Gilliland, 1968). One group of nine subjects was given allopurinol in a dose of 200 mg. twice d...

Many studies have reported proliferative, differentiating or protective effects of estradiol, notably through estrogen receptor alpha (ERα). On the contrary, the ligand-independent action of ERα is currently poorly documented notably in cell protection. The stable transfection of wild type, substituted or truncated form of ERα in PC12 cells (ERα negative cell line) lead the specific study of it...