We describe the complexation of isolated and native lipoproteins by natural macrocyclic polymers of glucose ("cyclodextrins"). Treatment of serum with cyclodextrin produces an immediate flocculation of the lipoproteins. The formation of cyclodextrin-lipoprotein complexes was evident on agarose electrophoresis gels. The concentration-dependent ability of alpha-cyclodextrin to precipitate chylomi...

Procedures are described for the isolation of lipoproteins from human serum by precipitation with polyanions and divalent cations. A mixture of low and very low density lipoproteins can be prepared without ultracentrifugation by precipitation with heparin and either MnCl(2) alone or MgCl(2) plus sucrose. In both cases the precipitation is reversible, selective, and complete. The highly concentr...

The feeding of cholesterol-rich diets alters the serum lipoproteins of a number of mammalian species. These lipoproteins are characterized by the presence of several classes of particles enriched in cholesteryl esters and apolipoprotein E (apo E). It was the aim of this study to determine whether one or more of these particles arises by de novo hepatic synthesis by characterizing nascent lipopr...

Lipoprotein profiles in experimental hypercholesterolemia were studied using euthyroid and hypothyroid cholesterol-fed rats. In both groups, serum cholesterol concentration increased, but to a lesser extent in the cholesterol-fed euthyroid rats, with similar changes in distribution among lipoprotein fractions in both groups. Agarose electrophoresis of plasma and individual lipoprotein fractions...

Serum and biliary lipoproteins, total cholesterol (Tc) and triglycerides (TG) were measured in patients with gallstones and in those without gallstones. Serum and biliary LDLc, TG and Tc were significantly higher (P < 0.001) in cases having gall stones than those without stones while HDLc were low (P < 0.001) in those with stones. No difference was found in very low density lipoproteins (VLDLc)...