We investigated the distribution of serotonin (5-HT)-containing neuroepithelial cells (NECs), the putative O(2) sensing cells, in the gills of four species of fish: trout (Oncorhynchus mykiss), goldfish (Carassius auratus), trairão (Hoplias lacerdae) and traira (Hoplias malabaricus) using immunohistochemical markers for 5-HT, synaptic vesicles and neural innervation. We found that all fish had ...

Some cells from cultured embryonic mouse hypothalamus were found to express aromatic-L-amino acid decarboxylase (EC 4.1.1.28) activity and serotonin uptake and storage. These neuron-like cells differed from serotoninergic neurons in cultured embryonic mouse brain stem since they did not contain tryptophan hydroxylase. We studied the effect of the serotonin agonist 8-hydroxy-2-[di-(n-propyl)amin...

The sequential application of the peroxidase-antiperoxidase (PAP) technique with nickel-intensified DAB and with DAB alone was used to visualize black peptide-immunoreactive endings on amber serotonin-immunoreactive cells in 1-2-micron paraffin sections of the hamster medulla. Met-enkephalin- and substance P-positive terminals were present on serotonin cells in the raphe nuclei, the ventral ret...

Serotonin plays an important role in the etiology of depression. Serotonin is also crucial for brain development. For instance, animal studies have demonstrated that early disruptions in the serotonin system affect brain development and emotion regulation in later life. A plausible explanation is that environmental stressors reprogram the serotonin system through epigenetic processes by alterin...

We studied binding of serotonin to protein(s) derived from rat basophil leukemia (RBL) cells and mast cells. We found two types of serotonin binding protein in RBL cells. These proteins differed from one another in molecular weight and eluted in separate peaks from sephadex G-200 columns. Peak I protein (Kr) = 1 .9 X 10-6 M) was a glycoprotein that bound to concanavalin A (Con A) ; Peak II prot...

Primary cultures of rat choroid plexus epithelial cells were established and used to investigate the role of the choroid plexus in the synthesis and secretion of transferrin. Transferrin gene expression was determined by a Northern blot analysis with a transferrin cRNA probe. A single transferrin mRNA species was detected and found to be the same size as the transcripts in the liver and Sertoli...

BACKGROUND Small bowel transplantations (SBT) are being increasingly performed to treat patients with irreversible intestinal failure or short-bowel syndrome. Histologic evaluation of small bowel allograft biopsies is important for the diagnosis of acute cellular rejection (ACR). Serotonin (5-hydroxytriptamin) is a biogenous amine of which up to 95% is present in the enterochromaffin cells of t...

In the central nervous system, serotonin, an important neurotransmitter and trophic factor, is synthesized by both mast cells and neurons. Mast cells, like other immune cells, are born in the bone marrow and migrate to many tissues. We show that they are resident in the mouse brain throughout development and adulthood. Measurements based on capillary electrophoresis with native fluorescence det...

Serotonin is best known as a neurotransmitter in the brain. Released by one neuron and picked up by another, it regulates mood and aggression. Many antidepressants, and the party drug Ecstasy, increase serotonin stimulation. But it is found outside the central nervous system as well, including in the pancreas, specifically in the beta cells that release insulin to regulate blood glucose levels....