Results: There were no significant differences in semen quality associated with the shape of the centrifugation tube either for refrigerated (5 ̊C) or thawed semen. The g-force of centrifugation did affect semen quality. In refrigerated samples centrifuged at 250g, the percentage of sperm with normal apical ridges (NAR) was lower (P <.01) and the percentage of damaged acrosomes was higher (P <.0...

Artificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability of spermatozoa in diluted semen depends on several factors, such as the interaction with the type of extender and storage duration. The aim of this study was to evaluate the effects of extenders and storage time on the motility and ultrastructure of cooled-preserved boar spermatozoa. Seme...

Animal origin egg yolk extender has been associated with microbial contamination and interference with microscopic examination. Therefore, replacement of such extender with plant origin compound is inevitable without compromising the desired qualities of an ideal extender. In the current experiment, we compared the effect of soy-based extender (25% and 50%) of five adult Holstein-Friesian bulls...

OBJECTIVES Although routinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of sperma...

BACKGROUND Long-chain polyunsaturated fatty acids (PUFAs) of the omega-3 family are important for sperm membrane integrity, sperm motility and viability. There are evidences to suggest that dietary supplementation with omega-3 fatty acids affects reproduction in men and males of different animal species. Therefore, the aim of current study was to investigate changes in the quality parameters of...

Aim: To investigate the prevalence of high levels of sperm DNA damage among men from infertile couples with both normal and abnormal standard semen parameters. Methods: A total of 350 men from infertile couples were assessed. Standard semen analysis and sperm chromatin structure assay (SCSA) were carried out. Results: Ninety-seven men (28% of the whole study group) had a DNA fragmentation index...

OBJECTIVE This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS Semen analysis and a sperm DNA fragmen...

Background: This study was carried out to evaluate the protective effects of a cell permeable antioxidant, Manganese (III) meso-tetrakis (N-ethylpyridinium-2-yl) porphyrin chloride (MnTE), alone or in combination with different concentration of Catalase on motility of frozenthawed goat semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from 3 goats, mixed...

BACKGROUND Although routinely used in assisted reproductive technology, human sperm cryopreservation is not an entirely successful procedure. This study determined the effect of nerve growth factor (NGF) supplementation of cryopreservation medium on post-thaw viability, motility, intracellular nitric oxide (NO) concentration, and DNA fragmentation of human spermatozoa in asthenozoospermic men. ...

This study assessed the effects of common extenders and diluents on the spermatozoa of African catfish (Clarias gariepinus), with the intent of obtaining a semen preservation protocol that can serve as a means of making fingerlings available to fish farmers all year round. Semen samples (milt) were pooled from mature broodstock males and pre-extension qualities were evaluated. Egg yolk, tomato ...