tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The paper on sperm DNA fragmentation by Ashok Agarwal et al. summarizes the currently available DNA fragmentation tests and discusses the role of sperm function tests in the intracytoplasmic sperm injection (ICSI) era (1). The authors have summarized the clinical utility of sperm DNA fragmentation test and have provid...

OBJECTIVE To determine which genetic tests are being performed on sperm donor applicants in the United States. DESIGN An electronic survey was distributed to 26 sperm banks to evaluate their genetic testing practices. SETTING Sperm banks in the United States. PATIENT(S) Not applicable. INTERVENTION(S) None. Survey of current practices. MAIN OUTCOME MEASURE(S) Survey of current practic...

may have been influenced by the presence of M540 bodies. M540 bodies are indeed mysterious structures. They stain positively with merocyanine, a fluorescent membrane probe normally associated with the measurement of membrane potential and disorder, and also generate signals in the TUNEL assay, even though these structures do not stain with DNA sensitive fluorochromes such as YOPRO-1 (Marchiani ...

the aim of this study was to evaluate the effects of the addition of antioxidants (vitamin e, β- mercaptoethanol and their combination) to semen extender.  progressive motility, viability and ph of fresh ram semen were examined. ejaculate samples were collected with artificial vagina from three ghezel-merinose rams (3 years old) during non- breeding reproduction season. semen was diluted with a...

Background: Since cellular membranes play key role in the physiological pathways of the cell, such as fertilization through almost every cellular function to cell division, it is very important to assess the structural and functional activity of the sperm membrane. In this study, a new method for measurement of sperm membrane stability measured the amount of zinc which liberated after being exp...

BACKGROUND Most studies on disclosure of mode of conception after fertility treatment have focused on donor insemination. We present a large, longitudinal cohort study of fertility patients who conceived through a variety of fertility treatments, including both non-donor and donor techniques. METHODS A cohort of 2812 women and men (n = 1406 couples) received questionnaires when initiating fer...

The sperm quality index (SQI) is predictive of fresh semen quality. Our objective was to examine if semen storage affects the SQI obtained from undiluted semen, or semen diluted with either Beltsville Poultry Semen Extender (BPSE) or Minimum Essential Medium (MEM) and held for 8 h at 4, 21, or 41 C. Dead o sperm percentage was higher and SQI was lower from undiluted versus diluted semen. Dead s...

The present study was aimed to observe the postthaw quality of Nili-Ravi buffalo bull semen cryopreserved during heat stress (hot summer months) and normal climatic conditions (fall, winter, spring months). For this purpose, four (n = 4) adult regular donor bulls were selected collect cryopreserve their throughout period an entire year. cryopreservation done following standard freezing protocol...

Purpose: To determine the clinical relevance of determining the semen pH during semen analysis as per WHO (World Health Organisation) standard criteria among the infertile couple attending a tertiary ART (Assisted Reproductive Technology) Centre in South India. Methods: Regular Semen analysis as per WHO standards (2010) Results: The Semen pH value showed no major correlation with other Semen pa...