PURPOSE We review the history of the cryopreservation of human sperm. The current methods of cryopreservation and indications are highlighted, and the success rates of the various uses of cryopreserved sperm are reviewed. Potential adverse sequelae of human cryopreservation are also discussed. MATERIALS AND METHODS Indications for cryopreservation include several applications, namely homologo...

BACKGROUND Cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been de- scribed for activation of a temporary response in ooc...

BACKGROUND AND OBJECTIVE Freezing changes sperm integrity remarkably. Cryopreservation involves cooling, freezing, and thawing and all these contribute to structural damage in sperm, resulting in reduced fertility potential. Low-level laser irradiation (LLLI) could increase energy supply to the cell and cause reactive oxygen species reduction (ROS), contributing to the restoration of oxygen con...

Semen traits and factors affecting sperm cryopreservation were assessed in the Iberian lynx (Lynx pardinus), a species regarded as the most endangered felid in the world. For cryopreservation, semen was washed, resuspended in a Tes-Tris-based diluent (TEST) or a Tris-based diluent (Biladyl), both with 20% egg yolk and 4% glycerol, loaded into straws, cooled to 5 degrees C using an automated pro...

Cryopreservation of semen is the most widely applied reproductive biotechnological tool used for rapid genetic improvement of livestock through artificial insemination, but the process of cryopreservation and thawing inflicts certain damage to spermatozoa plasma membrane and acrosome. This damage includes swelling and breakage of plasma membrane, loss of membrane selective permeability, change ...

The improved survival in recent years of young males suffering from cancer, and an understanding of the gonadotoxic effects of chemotherapy treatment, have motivated patients and clinicians to preserve fertility potential before embarking on adjuvant therapy. Among 231 men (mean age 28.0; range 15-56 years) diagnosed with malignant disease and referred to our unit for semen cryopreservation, 11...

Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreser...

Background: Cryopreservation has adverse effect on human spermatozoa via exposure to physical and chemical stress that may lead to alter in reactive oxygen species (ROS) levels. During sperm preparation or cryopreservation, centrifugation as a physical stress may effect on sperm quality probably with stress oxidative production and membrane damage. Therefore the aim of this study was to evaluat...

Semen cryopreservation is an import toll for reproductive management of fish species, which increasingly it is applied in the commercial production of fish. Cryopreservation need of the cryoprotectors agents (CPAs) that may be a complication factor in DNA extraction that can affect the quality of isolated DNA. Genomic DNA constitutes the total genetic information of an organism and it is used f...