Scalar coupling constants and signal splitting patterns in NMR spectra contain a wealth of short-range structural information. The extraction of these parameters from (1)H NMR spectra is often prohibited by simultaneous scalar coupling interactions with several other protons. Here we present a high-resolution NMR experiment where scalar coupling to only one selected signal is visible. All other...

A simple and rapid microextraction procedure based on dispersive liquid-liquid microextraction (DLLME) coupled with gas chromatography-flame ionization detection (GC-FID) was developed for the extraction and analysis of 2-phenylethanol in rose water sample. In the proposed approach, carbon tetrachloride and ethanol were used as extraction and dispersive solvents, respectively. Some important pa...

Electric arc furnace dust (EAFD) of ferromanganese production units, in the form of slurry, contains tar, alkalies, manganese, zinc, iron, silica, calcium, aluminum and other elements. A hydrometallurgical route based on solvent extraction technique was investigated for selective separation of manganese from the dust. Leaching of the EAFD resulted in an aqueous feed containing 4 g/L of manganes...

to extract active components from the traditional Chinese medicine Polygonum cuspidatum using a trans-resveratrol imprinted polymer. Good separation and purification of trans-resveratrol and emodin from the Polygonum cuspidatum extract are achieved after condition optimization. The extraction recoveries are 83% and 99% for trans-resveratol and emodin, respectively. The results show that the mol...

In this study, solid-phase microextraction fibers coated with modified silica particles (5 pm dp) bonded to methyl (C1), hexyl (C6), octyl (C8), and polymeric octadecyl (C18) groups are prepared and evaluated. Selective extraction of polycyclic aromatic hydrocarbons (PAHs) from river water is used to demonstrate the selective behavior of the fibers as a function of the alkyl chains bonded to th...

In  the  current  study,  a  new  technique  was  developed  for  quantification  and  qualification  of pseudoephedrine hydrochloride (PSE) in a real sample, which was based on electrochemical sensors and  molecular  imprinted  polymer  (MIP).  The  carbon  paste  electrode  (CPE)  was  modified  and optimized by a different ratio of MIP. MIP/CPE was used as the extraction and working electrod...

electric arc furnace dust (eafd) of ferromanganese production units, in the form of slurry, contains tar, alkalies, manganese, zinc, iron, silica, calcium, aluminum and other elements. a hydrometallurgical route based on solvent extraction technique was investigated for selective separation of manganese from the dust. leaching of the eafd resulted in an aqueous feed containing 4 g/l of manganes...

A new Pb(II) imprinted mercapto functionalized Montmorillonite was synthesize by an easy one step reaction by combining a surface imprinting technique for selective phase extraction of Pb(II) prior to its determinations by flame atomic absorption spectroscopy. The modified sorbent was characterized by FTIR and studied for preconcentration and determination of Pb(II) in natural water samples. Th...

The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/ g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or MüllerKauffmann tetrathionate, aliquots were used for DNA extraction by three differe...

Laser-capture microdissection (LCM) is a technique that enables selective extraction of desired cells from heterogeneous tissues compatible with subsequent molecular analyses. The specific visualization of desired cell types prior to LCM is essential for achieving selective capture. We have developed rapid and selective staining protocols for LCM extraction of microvessels from human and rat br...