Actin and actin-binding protein (ABP) have recently been purified from human platelet cytoskeletons (S. Rosenberg, A. Stracher, and R.C. Lucas, 1981, J. Cell Biol. 91:201-211). Here, the effect of ABP on the sedimentation of actin was studied. When ABP was added to preformed F-actin filaments, it bound until a maximum ratio of 1:9 (ABP:actin, mol:mol) was reached. however, when actin was polyme...

We apply a hybrid molecular dynamics and mesoscopic simulation technique to study the steady-state sedimentation of hard sphere particles for Peclet number (Pe) ranging from 0.08 to 12. Hydrodynamic backflow causes a reduction of the average sedimentation velocity relative to the Stokes velocity. We find that this effect is independent of Pe number. Velocity fluctuations show the expected effec...

In order to try to improve the results of direct smear microscopy, we used the mucus-digesting quality of chitin in tuberculosis (TB) laboratories. For this purpose, a total of 430 sputum specimens were processed by the N-acetyl-L-cysteine concentration, sodium hypochlorite (NaOCl) liquefaction, chitin sedimentation, and direct microscopy methods. Then, the smear sensitivity for acid-fast bacil...

Sediment particles originating from erosion processes in the catchment are propagated along with the river flow. When the flow of a river is stored in a reservoir, the sediment settles in the reservoir and reduces its capacity. Reduction in the storage capacity of a reservoir beyond a limit hampers the purpose for which it was designed. Thus assessment of sediment deposition becomes very import...

1. Fibrin clots prepared in the absence of calcium can be dissolved in solutions of lithium chloride and bromide and sodium bromide and iodide, as well as of guanidine hydrochloride and urea. These salts do not denature fibrinogen under the same conditions of concentration, temperature, and time. Sedimentation experiments on the fibrin solutions show in each case a single sharp peak with a sedi...

In a previous publication it was pointed out that definite relationships existed between the growth curve of a 100 per cent transplantable methylcholanthrene-induced hamster sarcoma and several blood cell factors (1~). As the tumor grew in the hamster cheek pouch, characteristic alterations were noted in the levels of hemoglobin, red and white blood cells, differential white blood cell counts, ...

Analytical ultracentrifugation allows one to measure in real-time the concentration gradients arising from the application of a centrifugal force to macromolecular mixtures in solution. In the last decade, the ability to efficiently solve the partial differential equation governing the ultracentrifugal sedimentation and diffusion process, the Lamm equation, has spawned significant progress in t...

Magnetic resonance imaging was employed for the first time to visualize gravity sedimentation of kaolin suspensions, allowing internal spatial distribution during be investigated.