نتایج جستجو برای: sectioned
تعداد نتایج: 4965 فیلتر نتایج به سال:
Thin sections of polyhedra obtained from gipsy moth larvae infected with P. dispar virus and from silkworm larvae infected with B. mori virus revealed viral particles contained within a pseudohexagonal, macromolecular, paracrystalline lattice. The gipsy moth virus occurs in bundles of one to eight rods enclosed by a limiting membrane. The particles of the silkworm virus, although generally occu...
We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an opti...
We describe a method of obtaining optical sectioning with a standard wide-field fluorescence microscope. The method involves acquiring two images, one with nonuniform illumination (in our case, speckle) and another with uniform illumination (in our case, randomized speckle). An evaluation of the local contrast in the speckle-illumination image provides an optically sectioned image with low reso...
Immunocytochemistry on sectioned tissue is a powerful method for determining the cellular and subcellular distributions of an epitope. However, where the expression is patterned, understanding the distribution often requires extensive, time-consuming threedimensional reconstruction with all its attendant artifacts. The techniques described in this unit circumvent these problems. The distributio...
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