Synthetic block copolymers comprising two or more compositionally distinct chains have attracted significant attention due to their ability to self-assemble into ordered microstructures. Although tremendous progress has been made in the chemical synthesis of polymers, the unsurpassed degree of control and diversity of monomers combined with advances in recombinant DNA technology permits the syn...

Epitope tagging has proven a useful method for monitoring recombinant proteins expressed in either prokaryotic or eukaryotic cells. For this purpose, the epitope should not be present on any of the host cell’s proteins, it should not interfere with either structure or function of the tagged protein, and the corresponding antibody must be of high specificity and affinity. To this day, only a few...

Several techniques in molecular biology frequently yield partial and ambiguous data on genes and gene products. For instance, N-terminal sequence analysis of oligopeptide cleavage products generates this type of sequence data. Typically, data generated from blotted or HPLC-resolved peptides consist of disconnected and unordered oligopeptides derived from N-terminal analysis of fragments resulti...

For several reasons, it can be desirable to probe a filter obtained after Western blotting of polyacrylamide gels with different antibodies. On the one hand, protein amounts may be limiting and re-probing of a protein filter may be the only way to identify different antigens. On the other hand, in the case of two-dimensional (2-D) gel electrophoresis, exact reproducibility of the protein patter...

Background: We have earlier shown that exposure of human endothelial cell line EA.hy926 to 900 MHz GSM mobile phone radiation causes changes in the expression of numerous proteins. Here, we have examined the effects of 1800 MHz GSM mobile phone signal on the proteome of the same cell line. Results: EA.hy926 cells were exposed for one hour to 1800 MHz GSM signal, simulating mobile phone talking ...

The association of membrane proteins with a single transmembrane a-helix (TMH) has been shown to play a critical role in numerous cellular processes. For example, TMH dimerization of the ErbB family of receptor tyrosine kinases (RTKs) results in trans-phosphorylation and subsequent activation of the downstream pathways of growth factor signalling. A series of naturally occurring, single amino a...

Band 3 (anion exchanger 1, AE1) is the most abundant integral protein of the red blood cell membrane. It is composed of 911 amino acids encoded by the EPB3 gene. Several mutations in the EPB3 gene have been found associated to different diseases, eg, hereditary spherocytosis, Southeast Asian ovalocytosis, chorea-acanthocytosis, and familial distal renal tubular acidosis, thus demonstrating that...

Nature has provided us with a range of reactive nanoscale platforms, in the form of protein cage architectures such as viral capsids and the cages of ferritin-like proteins. Protein cage architectures have clearly demarcated exterior, interior, and interface surfaces consisting of precisely located chemical functionalities. In the present work, we demonstrate that the small heat shock protein (...

Human Lp[a] was isolated in preparative amounts from two donors; the native lipoprotein and its constituent apoproteins, apo[a] and apoB, were characterized extensively. Based on differences in apparent molecular weight, four different isoforms of apo[a], a,-%, were observed between the two donors. The number and relative distribution of these isoforms varied between donors but were constant fo...

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