نتایج جستجو برای: scratch assay
تعداد نتایج: 229665 فیلتر نتایج به سال:
Blue-green alga (Spirulina platensis) is a well renowned nutri-supplement due to its high nutritional and medicinal properties. The aim of this study was to examine the wound healing efficiency of Spirulina platensis at various solvent extracts using in vitro scratch assay on human dermal fibroblast cells (HDF). Various gradient solvent extracts (50 μg/ml of methanolic, ethanolic and aqueous ex...
AIM Spinal cord injury (SCI) is a serious condition of the central nervous system and it affects the quality of life and even hampers the day-to-day activity of the patient. In the current study, we investigated the efficacy of intrathecal administration of flavopiridol in an experimental animal model of SCI. The study also aimed at exploring the physiological effects of flavopiridol on neurons...
This study investigated the effect of cerium chloride (CeCl₃) on cell migration and gene expression of human foreskin fibroblasts (HFF). HFF were exposed to three different CeCl₃ solutions (1%, 5% and 10%, w/v %) for three different time durations (1, 5 and 10 min). 72 h after exposure to CeCl₃, cell viability was assessed by MTT test. A scratch-wounded assay determined the cell migration and t...
MicroRNAs have been implicated in cancer cells proliferation, migration and invasion, including clear-cell renal cell carcinoma (ccRCC). DNA methylation, a major epigenetic change associated with cancer, may lead to transcriptional silencing of tumor suppressor genes, including miRNAs, which may be a possible mechanism in carcinogenesis. In this study, we aim to investigate the role of miR-10b ...
Combinations of anticancer agents are likely to be more effective in breast cancer therapy. Hence, in the present study we evaluated the in vitro anticancer activity of brucine when combined with gemcitabine in MDA MB-436 human breast cancer cells. The cells were cultured in vitro and treated with brucine and gemcitabine combinations. Cell viability was assessed by trypan blue exclusion assay a...
need to monitor disappearance and formation of reaction components. Such an assay would require adequate resolution of the reaction species, sufficient sensitivity to quantitate the dwindling component, and typically, the ability to accommodate various sample loads. It might not be necessary to start from scratch and set out on a comprehensive method development project, as one might be able to...
Quantifying the impact of biochemical compounds on collective cell spreading is an essential element of drug design, with various applications including developing treatments for chronic wounds and cancer. Scratch assays are a technically simple and inexpensive method used to study collective cell spreading; however, most previous interpretations of scratch assays are qualitative and do not pro...
The present study detected p75 neurotrophin receptor (p75NTR) expression in tongue squamous cell carcinoma (TSCC) cell lines, in order to define the biological properties of p75NTR+ cells and to confirm the use of p75NTR+ as a surface marker for TSCC stem cells. p75NTR+ cells were separated from Tca‑8113 and CAL‑27 TSCC cells by fluorescence‑activated cell sorting. Colony formation, MTT and scr...
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