This project is inspired by the recent development of the Cap analysis gene expression (CAGE) method, which introduces new benefits to standard gene expression techniques such as being higher-throughput and more accurately mapping the transcription start sites.7 Building off of previous serial analysis of gene expression (SAGE) methods, I aim to extract biological meaning from the gene expressi...

In this study, it was aimed to determine the anti-bacterial activities of plant essential oils such as thyme, rosemary, sage, linden, black seed, bay leaf, peppermint, parsley, cinnamon and oleagen on vancomycin resistant enterococci (VRE) pathogenic microorganisms clinical importance. inhibition zones extract 10 plants prepared at 2.5 5 mg / mlconcentrations VRE various were detected by Disk D...

Encapsulated Salvia officinalis L. powder (SESP) was developed from extract obtained by supercritical fluid extraction (SFE), and it compared with commercial sage of hydro-distillation (HESP). The optimised conditions 30 MPa at 50 °C for laboratory scale SFE were found to generate the highest yield 40.96 ± 0.84%. later applied in upscaled extraction, which yielded about 30.10 1.33% extract. Lar...

The SAGE (serial analysis of gene expression) method is sensitive at detecting the lower abundance transcripts. More than a third of human SAGE tags identified are novel representing the low abundance unknown transcripts. Using the GLGI method (generation of longer 3’ EST from SAGE tag for gene identification), we converted 1,009 low-copy, human X chromosomespecific SAGE tags into the 3’ ESTs. ...

We have developed a technique called the generation of longer cDNA fragments from serial analysis of gene expression (SAGE) tags for gene identification (GLGI), to convert SAGE tags of 10 bases into their corresponding 3' cDNA fragments covering hundred bases. A primer containing the 10-base SAGE tag is used as the sense primer, and a single base anchored oligo(dT) primer is used as an antisens...

BACKGROUND Although Salvia sclarea (clary sage) is widely used in aromatherapy and has antioxidant and antimicrobial properties, its mechanisms of action remain poorly understood. We therefore assessed whether clary sage is effective in treating endothelial dysfunction induced by chronic immobilization stress in rats. METHODS Rats were intraperitoneally injected with almond oil, clary sage oi...

Coyotes (Canis latrans) are lethally controlled throughout the range of Greater Sage-Grouse (Centrocercus urophasianus) and it has been suggested that such control may benefit sage-grouse. However, the perceived benefits of control are based on the direct effects of coyotes on sage-grouse and largely ignore potential indirect interactions. Here, we summarize some of the evidence for direct effe...