A eukaryotic formate dehydrogenase (EC 1.2.1.2, FDH) with its substrate specificity changed from NAD(+) to NADP(+) has been constructed by introducing two single-point mutations, Asp(196)-->Ala (D196A) and Tyr(197)-->Arg (Y197R). The mutagenesis was based on the results of homology modelling of a NAD(+)-specific FDH from Saccharomyces cerevisiae (SceFDH) using the Pseudomonas sp.101 FDH (PseFDH...

The amino acid sequences of haemoglobin-like proteins from the bacteria Alcaligenes eutrophus, Bacillus subtilis, Erwinia chrysanthemi, Escherichia coli, Vibrio parahaemolyticus, Vitreoscilla sp. and the yeast Saccharomyces cerevisiae were studied. Phylogenies based on distance and parsimony analysis showed that the eubacterial group can be easily distinguished from the other haemoglobin-like p...

The fungal cell wall viewed through the electron microscope appears transparent when fixed by the conventional osmium tetroxide method. However, ruthenium tetroxide post-fixing has revealed new details in the ultrastructure of Penicillium sp. hyphae and Saccharomyces cerevisiae yeast. Most significant was the demonstration of two or three opaque diverse electron dense layers on the cell wall of...

The microflora of Tibetan kefir grains was investigated by culture- independent methods. Denaturing gradient gel electrophoresis (DGGE) of partially amplified 16S rRNA for bacteria and 26S rRNA for yeasts, followed by sequencing of the most intense bands, showed that the dominant microorganisms were Pseudomonas sp., Leuconostoc mesenteroides, Lactobacillus helveticus, Lactobacillus kefiranofaci...

The screening of antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, yeast and fungi was carried out on isopropanol extracts prepared from 121 isolates of endophytic fungi isolated from medicinal plants in Malaysia. Sensitivity was found to vary among the microorganisms. Bacillus subtilis, Saccharomyces cerevisiae and Alternaria sp. were susceptible to extracts from t...

Bioreduction of several prochiral carbonylic compounds such as acetophenone (1), ethyl acetoacetate (2) and ethyl phenylpropionate (3) to the corresponding optically active sec-alcohols 1a-3a was performed using wild-type strains of Pichia pastoris UBB 1500, Rhodotorula sp., and Saccharomyces cerevisiae. The reductions showed moderate to excellent conversion and high enantiomeric excess, in an ...

Mexico is among the most important citrus fruit producers in world. However, during storage, several problems related to fungi can arise. The common fungal postharvest diseases detected on Citrus limon var Eureka (Italian lime) produced Tamaulipas state are green/blue mold (Penicillium spp.), fusarium rot (F. oxysporum, F. solani, proliferatum, others), and anthracnose (Colletotrichum spp.). In...

Stingless bee honey is a nutritious food that contains variety of vitamins, minerals, enzymes, and antioxidants. It known to have higher nutritional medicinal properties compared produced by other species. Cider well-known functional drink high antioxidants, which can help protect against cellular damage caused free radicals. This study aimed investigate the potential co-fermentation with yeast...

This study aimed to investigate anaerobic co-digestion of palm oil mill effluent (POME) with cassava peels (CP) and cabbage waste (CW) for biomethane production. The digestion (AD) in 10L capacity bioreactors loaded separately three different ratios (3L/ 520g, 3L/ 600g 680g) POME/CP, POME/CW POME only (control) was operated under ambient temperature (25 - 36⁰C) pH range 6.5 8.5 45 days. Standar...