Since Saccharomyces cerevisiae appears to be an emerging pathogen, there is a need for a valuable molecular marker able to distinguish among strains. In this work, we investigated the potential value of microsatellite length polymorphism with a panel of 91 isolates, including 41 clinical isolates, 14 laboratory strains, and 28 strains with industrial relevance. Testing seven polymorphic regions...

Based on experimental evidence in animals showing that the oral administration of Saccharomyces boulardii is effective in reducing morbidity and mortality due to Clostridium difficile-induced pseudomembranous colitis, we conducted an open trial to examine the effects of the living yeast, given as primary therapy, in a selected group of infants and children with persistent intestinal symptoms re...

Intestinal amebiasis has a worldwide distribution and is common in tropical and subtropical areas. [n this prospective 7-month study, we studied the main clinical findings in patients with acute amebic proctocolitis and the efficacy of Saccharomyces boulardii (SB) treatment of these patients.Initially, 57 cases with acute amebic proctocolitis were selected. The maximum occurrence of infecti...

Main results 9 trials (1214 patients) met the selection criteria. 2 of the trials studied children. No statistical heterogeneity or publication bias was detected among the 9 trials. The pooled odds ratio (OR) showed that probiotic treatment was more effective than placebo in the prevention of diarrhoea (OR 0.37, 95% CI 0.26 to 0.53) (table). 4 trials that used S. boulardii (yeast trials) also f...

abstract: this study was aimed at probiotication of tomato and carrot juices using lactobacillus plantarum, lb. fermentum, lb. casei, lysinibacillus sphaericus and saccharomyces boulardii. to enhance the stability, the probiotic cultures were micro-encapsulated using alginate coated chitosan beads. tomato and carrot juice samples were pasteurized for 20 min at 63 °c. lb. fermentum, lb. plantara...

Background: Diarrhoea exerts an enormous toll in terms of mortality, morbidity, social inconvenience, loss of work productivity, and consumption of medical resources. Acute infectious diarrhea remains one of the most common causes of mortality in developing countries, particularly among children, accounting for 2-3 million deaths per year. Aim: To compare the contractile effect of a synthetic a...

BACKGROUND Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small GTP-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of MAPKs and nuclear translocation of NF-kappaB. Worldwide interest in the use of functional foods containing probiotic bacteria for heal...

The well-known probiotic GRAS Saccharomyces boulardii (CNCM I-745) was used for the first time to produce glutathione (GSH). culture conditions affecting GSH biosynthesis were screened using a Plackett–Burman design (PBD). Analyzing regression coefficients 12 tested variables, yeast extract, glucose, peptone, cysteine, temperature and agitation rate had positive significant effect on production...