One cause of a reduction in the efficiency of feed utilisation in the rumen is the non-specific lysis of bacteria within the rumen and subsequent fermentation of the bacterial protoplasm. Bacteriophages are implicated in this lysis, are obligate pathogens of bacteria and occur in dense populations in the rumen. Large numbers are present (up to lo*’ per millilitre of fluid) in the rumen. These v...

This study was conducted to investigate effects of disodium fumarate (DF) on fermentation characteristics and microbial populations in the rumen of Hu sheep fed on high-forage diets. Two complementary feeding trials were conducted. In Trial 1, six Hu sheep fitted with ruminal cannulae were randomly allocated to a 2 × 2 cross-over design involving dietary treatments of either 0 or 20 g DF daily....

S TUDIES by Appleby et al. (1956), Gutierrez and tlungate (1957), Eadie et al. (1959) and Eadie and Hollson (1962) indicated that ciliate protozoa normally inhabiting the rumen exerted considerable influence upon end products of rumen fermentation and upon tureen bacterial populations. The full significance of protozoa to the nutrition of cattle and sheep is not clear. However, Christiansen et ...

A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of ingesta) of lactate-utilizing bacteria to be made with a high degree of specificit...

The aim of this study was to detect the major bacteria present in rumen microbiota. Here, we performed qPCR based absolute quantitation of selected rumen microbes in rumen fluid of river buffalo adapted to varying proportion of concentrate to roughage diets. Animals were adapted to roughage-to-concentrate ratio in the proportion of 100:00 (T1), 75:25 (T2), 50:50 (T3) and 25:75 (T4) respectively...

Twenty-nine strains of 14 species of rumen bacteria were screened for their ability to hydrolyse Ala2, Ala5, GlyArg-4-methoxy-2-naphthylamide (GlyArg-MNA) and Leu-MNA. Several species, notably Megasphaera elsdenii, were active against Ala2, and a smaller number, including Bacteroides ruminicola, Butyrivibrio fibrisolvens, Ruminococcus flavefaciens, Lachnospira multipara and Ruminobacter amyloph...

Bioavailabilities of oral rumen-protected and non-protected formulations of sulfamethoxazole (SMS) were compared in ruminating calves, since in vitro degradation of SMX in ruminal fluid was confirmed. The coated with a gastric-acid-soluble polymer and uncoated formulations were administered to 3 calves through a catheter. Neither formulation could produce sufficient blood concentration of the d...

BACKGROUND: The use of antibiotics as feed additive in animal feeds due to the appearance of residues in milk and meat and their effects on human health has restricted. Two of essential oils with high potential for use in ruminant diet are Mentha piperita (peppermint) and Mentha pulegium (pennyroyal) essential oil. OBJECTIVE: This study was conducted to investigate the effects of essential oils...

In experiments on digestive function in ruminants, markers can be used to estimate the rate of movement of digesta, the volume of a viscus and the rates of absorption of solutes from the gut. The most widely used water-soluble marker is polyethylene glycol (PEG), which was introduced by Sperber, HydCn & Ekman (1953). They found that the polymer with mean molecular weight 4000 was not degraded i...

the effect of the substitution of imported raw materials (corn and soyabeans) by local food resources (barley, white sorghum, triticale and horse bean) on the parameters of faciesfermentation and digestibility in the rumen of sheep was evaluated. four sicilo-sarde rams 4.8±0.5 years of age with an average live weight of 45.25±3.5 kg, permanently canulated in the rumen and housed in individual c...