نتایج جستجو برای: rt pcr nested pcr

تعداد نتایج: 222624  

2017
Anita M. Shete Pragya Yadav Vimal Kumar Tushar Nikam Kurosh Mehershahi Prasad Kokate Deepak Patil Devendra T. Mourya

BACKGROUND & OBJECTIVES Bats are recognized as important reservoirs for emerging infectious disease and some unknown viral diseases. Two novel viruses, Malsoor virus (family Bunyaviridae, genus, Phlebovirus) and a novel adenovirus (AdV) (family, Adenoviridae genus, Mastadenovirus), were identified from Rousettus bats in the Maharashtra State of India. This study was done to develop and optimize...

Journal: :The Journal of molecular diagnostics : JMD 2006
Kamaljit Singh Ajit Lale Eng Eong Ooi Lily-Lily Chiu Vincent T K Chow Paul Tambyah Evelyn S C Koay

Laboratory testing for dengue virus is used to confirm the diagnosis of dengue virus infection and to differentiate dengue from other febrile tropical illnesses. There are few data on the clinical use of reverse transcription-polymerase chain reaction (RT-PCR) for diagnosis of dengue virus infection. We prospectively evaluated 121 consecutive patients with possible dengue who had samples submit...

Journal: :Journal of clinical microbiology 2005
Manfred Weidmann P Schmidt M Vackova K Krivanec P Munclinger F T Hufert

A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.

Journal: :Jundishapur Journal of Microbiology 2022

Background: Fast and precise detection of SARS-CoV-2 RNA in clinical samples subsequent quarantine are two critical factors preventing virus transmission distribution through the community. The false-negative result is a major problem because kind sample (swab sample), sampling error, sensitivity PCR test, which can be reduced by much more sensitive test such as nested PCR. Objectives: This stu...

Fadyia Mahdi Alameedy, Ghaida Raheem Lateef Al-Awsi, Saade Abdalkareem Jasim, Shireen Ali Obaid,

Background: Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disorder (EBV-PTLD) is a known and serious condition for the hematopoietic stem cell transplantation. This study evaluated the prevalence of EBV in umbilical cord samples of healthy pregnant women in Baghdad, Iraq.  Methods: 800 umbilical cord blood (UCB) samples were collected from healthy pregnant women. The ...

Journal: :Veterinary research 2000
V Legay C Sailleau G Dauphin S Zientara

The highly neurotropic Borna Disease Virus (BDV), which belongs to the Mononegavirales order--Bornaviridae family--is generally detected using the RT-nested-PCR. If false positive results (often caused by laboratory contaminations) can be avoided, some false negative results which are mostly due to inhibitory effects of some reaction components and/or to sample preparation errors, can occur. Th...

Journal: :iranian journal of microbiology 0
hashem radmehr health research institute, infectious and tropical diseases research center; ahvaz jundishapur university of medical sciences, ahvaz, iran manoochehr makvandi health research institute, infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, iran virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran alireza samarbafzadeh health research institute, infectious and tropical diseases research center; ahvaz jundishapur university of medical sciences, ahvaz, iran. virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran ali teimoori 2virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran niloofar neisi 2virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran mojtaba rasti 2virology department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran

background and objectives: hepatitis c virus (hcv) is a major public health problem worldwide. replication and persistence of hcv genome have been described in the liver tissue as well as b cells lymphocyte. several investigations have reported that long-term persistence of hcv in b cells may result in hodgkin and non-hodgkin lymphoma. this study was aimed to determine frequency of hcv rna in h...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه صنعتی اصفهان - دانشکده کشاورزی 1390

ویروس برگ قاشقی گیلاس(clrv) cherry leaf roll virus از جنس nepovirus و خانواده comoviridae، دارای گسترش جهانی می باشد. این ویروس به طور طبیعی دامنه وسیعی از گیاهان علفی و چوبی را آلوده می کند که مهم ترین آنها متعلق به جنس های betula،fagus ، juglans و prunus می باشند. ویروس برگ قاشقی گیلاس، نارون، شاه توت، تمشک، گردو، زغال اخته، درختان میوه هسته دار و دیگر درختان و بوته ها را آلوده کرده و خسارت ش...

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