نتایج جستجو برای: rrna gene

تعداد نتایج: 1153532  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1996
T Tenson A DeBlasio A Mankin

A pentapeptide open reading frame equipped with a canonical ribosome-binding site is present in the Escherichia coli 23S rRNA. Overexpression of 23S rRNA fragments containing the mini-gene renders cells resistant to the ribosome-inhibiting antibiotic erythromycin. Mutations that change either the initiator or stop codons of the peptide mini-gene result in the loss of erythromycin resistance. No...

A. Nikbin A.R. Alborzi A.R. Ghadrdan M.R. Tabandeh S. Bahrami,

Considering the importance of Theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of T. equi among dromedaries in central Iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. For this purpose, a total of 161 blood samples from camels were examined in terms of T. equ...

The present study characterized Argulus spp. infecting the cultured carps using 18S rRNA gene sequences, estimated the genetic similarity among Argulus spp. and established their phylogenetic relationship. Of the 320 fish samples screened, 34 fish (10.6%) had Argulus infection. The parasitic frequency index (PFI) was observed to be high (20%) in Hypophthalmichthys molitrix and Labeo bata. The f...

2013
Jordan A. Fish Benli Chai Qiong Wang Yanni Sun C. Titus Brown James M. Tiedje James R. Cole

Ribosomal RNA genes have become the standard molecular markers for microbial community analysis for good reasons, including universal occurrence in cellular organisms, availability of large databases, and ease of rRNA gene region amplification and analysis. As markers, however, rRNA genes have some significant limitations. The rRNA genes are often present in multiple copies, unlike most protein...

Journal: :The Journal of Cell Biology 2008
Elaine Sanij Gretchen Poortinga Kerith Sharkey Sandy Hung Timothy P. Holloway Jaclyn Quin Elysia Robb Lee H. Wong Walter G. Thomas Victor Stefanovsky Tom Moss Lawrence Rothblum Katherine M. Hannan Grant A. McArthur Richard B. Pearson Ross D. Hannan

In mammals, the mechanisms regulating the number of active copies of the approximately 200 ribosomal RNA (rRNA) genes transcribed by RNA polymerase I are unclear. We demonstrate that depletion of the transcription factor upstream binding factor (UBF) leads to the stable and reversible methylation-independent silencing of rRNA genes by promoting histone H1-induced assembly of transcriptionally i...

2017
Khayalethu Ntushelo Joseph Mafofo

The draft genome of Pectobacterium carotovorum subsp. brasiliense (Pcb) which causes blackleg of potato was submitted to the NCBI and released with reference number NZ_LGRF00000000.1. The estimated genome size based on the draft genome assembly is 4,820,279bp from 33 contigs ranging in length from 444 to 1,660,019 nucleotides. The genome annotation showed 4250 putative genes, 4114 CDS and 43 ps...

Journal: :RNA 2012
Anna Y Golovina Margarita M Dzama Ilya A Osterman Petr V Sergiev Marina V Serebryakova Alexey A Bogdanov Olga A Dontsova

The ribosomal RNA (rRNA) of Escherichia coli contains 24 methylated residues. A set of 22 methyltransferases responsible for modification of 23 residues has been described previously. Herein we report the identification of the yhiR gene as encoding the enzyme that modifies the 23S rRNA nucleotide A2030, the last methylated rRNA nucleotide whose modification enzyme was not known. YhiR prefers pr...

Journal: :Journal of clinical microbiology 1999
A Guimaraes-Peres F Portaels P de Rijk K Fissette S R Pattyn J van Vooren P Fonteyne

Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA ge...

Journal: :Nucleic acids research 1982
H G Köchel H Küntzel

The alignment of gene sequences coding for A. nidulans mitochondrial L-rRNA and E. coli 23S rRNA indicates a strong conservation of primary and potential secondary structure of both rRNA molecules, except that homologies to the 5'-terminal 5.8S-like region and the 3'-terminal 4.5S-like region of bacterial rRNA are not detectable on mtDNA. The structural organization of the A. nidulans mt L-rRNA...

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