background: polychlorinated biphenyls (pcbs) are synthetic aromatic compounds containing two benzene rings with one to ten chlorine atoms. there are concerns about the health risks of these compounds in the environment due to their toxicity, persistence, and biological accumulation. pcbs wastes must be treated before being discharged to the environment because they will pollute the water, soil,...

The taxonomic position of an actinomycete, strain K07-23T, isolated from a cold desert of the Himalayas, India, was established by a polyphasic approach. The strain exhibited phenotypic characters that were typical of the genus Rhodococcus. 16S rRNA gene sequence (1467 bases) comparisons confirmed that strain K07-23T belongs to the genus Rhodococcus. 16S rRNA sequence similarity studies showed ...

Introduction. The genus Rhodococcus is a non-sporeforming, high GC Gram-positive bacterium, a member of phylum Actinobacteria. Research studies for Rhodococcus have been mainly focused on its great ability to degrade recalcitrant chemicals such as poly-chlorinated biphenyls and dioxins (1, 2). Also, their pathogenic features have been intensively studied in limited species. However, only a few ...

Rieske nonheme iron oxygenases form a large class of aromatic ring-hydroxylating dioxygenases found in microorganisms. These enzymes enable microorganisms to tolerate and even exclusively utilize aromatic compounds for growth, making them good candidates for use in synthesis of chiral intermediates and bioremediation. Studies of the chemical stability and thermostability of these enzymes thus b...

The ability to generate tagged mutants of Rhodococcus spp. will facilitate a deeper understanding of this medically and commercially important genus. The absence of efficient transposon systems in these organisms has here been overcome by the use of Tn5-based DNA-protein transposition complexes which can transpose at high efficiency. To achieve this, electroporation efficiencies and antibiotic ...

This review discusses the use of engineered microbes for bioconversion lignin from plant biomass to produce renewable chemicals. Existing bacterial hosts are discussed, such as Pseudomonas putida KT2440 and Rhodococcus jostii RHA1, case studies where they have been generate aromatic non-aromatic products described, different types substrates used these studies. Other bacteria identified degrade...

Lignin-derived (e.g. phenolic) compounds can compromise the bioconversion of lignocellulosic biomass to fuels and chemicals due to their toxicity and recalcitrance. The lipid-accumulating bacterium Rhodococcus opacus PD630 has recently emerged as a promising microbial host for lignocellulose conversion to value-added products due to its natural ability to tolerate and utilize phenolics. To gain...

Non-Scientific Abstract Throughout the past decade, AIDS has become one of the most threatening and prevalent diseases in America. As the number of patients suffering from the potentially fatal disease increases, so does the demand for different types of drug therapy. One of the more successful therapies is Crixivan, a drug produced by MERCK. Although highly effective at slowing the spread of ...

Rhodococcus erythropolis N9T-4, which was isolated from crude oil, showed extremely oligotrophic growth and formed its colonies on a minimal salt medium solidified using agar or silica gel without any additional carbon source. N9T-4 did not grow under CO(2)-limiting conditions but could grow on a medium containing NaHCO(3) under the same conditions, suggesting that the oligotrophic growth of N9...